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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Overview
Product name
Anti-COL1A2 antibody
See all COL1A2 products (4) ...
Description
Rabbit polyclonal to COL1A2
Specificity
ab72637 detects endogenous levels of fragment of activated COL1A2 resulting from cleavage adjacent to Gly1102.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from human COL1A2.
Positive control
Extracts from Jurkat cells treated with etoposide (25uM, 24hours).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Growth Factors/Hormones >> TGF
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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Western blot - COL1A2 antibody (ab72637)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 95 kDa (predicted molecular weight: 129 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Relevance
COL1A2 is the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Defects in COL1A2 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity.
Cellular localization
Secreted, extracellular space, extracellular matrix
Alternative names
- Alpha 2 collagen type I antibody
- Alpha 2 type I collagen antibody
- Alpha 2(I) collagen antibody
see all
Database links
- Entrez Gene: 1278 Human
- Omim: 120160 Human
- SwissProt: P08123 Human
Anti-COL1A2 antibody images:
Western blot - COL1A2 antibody (ab72637)
All lanes : Anti-COL1A2 antibody (ab72637) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells treated
with etoposide (25µM, 24hours)
Lane 2 : Extracts from Jurkat cells treated
with etoposide (25µM, 24hours) with Cl-peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 129 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Note: ab72637 detects a fragment of activated COL1A2 resulting from cleavage adjacent to Gly1102.
References for Anti-COL1A2 antibody (ab72637)
ab72637 has not yet been referenced specifically in any publications.
Publishing research using ab72637? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"