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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Overview
Product name
Anti-COL1A2 antibody
See all COL1A2 products (4) ...
Description
Rabbit polyclonal to COL1A2
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Rat, Cow, Dog
Immunogen
Synthetic peptide corresponding to a region within internal amino acids 1044 - 1093 (PGSVGPAGPR GPAGPSGPAG KDGRTGHPGT VGPAGIRGPQ GHQGPAGPPG) of Human COL1A2 (NP_000080)
PGSVGPAGPR GPAGPSGPAG KDGRTGHPGT VGPAGIRGPQ GHQGPAGPPG
Positive control
Fetal liver lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Growth Factors/Hormones >> TGF
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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Western blot - COL1A2 antibody (ab84713)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 129 kDa.
Target
Relevance
COL1A2 is the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Defects in COL1A2 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity.
Cellular localization
Secreted, extracellular space, extracellular matrix
Alternative names
- Alpha 2 collagen type I antibody
- Alpha 2 type I collagen antibody
- Alpha 2(I) collagen antibody
see all
Database links
- Entrez Gene: 403824 Dog
- Entrez Gene: 1278 Human
- Entrez Gene: 84352 Rat
- Omim: 120160 Human
- SwissProt: O46392 Dog
- SwissProt: P08123 Human
- SwissProt: P02466 Rat
Anti-COL1A2 antibody images:
Western blot - COL1A2 antibody (ab84713)
Anti-COL1A2 antibody (ab84713) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 129 kDa
Observed band size : 144 kDa (why is the actual band size different from the predicted?)
Additional bands at : 92 kDa. We are unsure as to the identity of these extra bands.
References for Anti-COL1A2 antibody (ab84713)
ab84713 has not yet been referenced specifically in any publications.
Publishing research using ab84713? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"