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Overview

  • Product nameAnti-COL1A2 antibodySee all COL1A2 primary antibodies ...
  • Description
    Rabbit polyclonal to COL1A2
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 1044 - 1093 (PGSVGPAGPR GPAGPSGPAG KDGRTGHPGT VGPAGIRGPQ GHQGPAGPPG) of Human COL1A2 (NP_000080)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlFetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceCOL1A2 is the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Defects in COL1A2 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity.
  • Cellular localizationSecreted, extracellular space, extracellular matrix
  • Database links
    see all
  • Alternative names
      Alpha 2 collagen type I antibodyAlpha 2 type I collagen antibodyAlpha 2(I) collagen antibody
      Collagen alpha 2(I) chain antibodyCollagen I alpha 2 polypeptide antibodyCollagen of skin tendon and bone alpha 2 chain antibodyCollagen type I alpha 2 antibodyOI4 antibodyOsteogenesis imperfecta type IV antibodyType I procollagen antibody
    see all

Anti-COL1A2 antibody images

  • Western blot - COL1A2 antibody (ab84713)
    Western blot - COL1A2 antibody (ab84713)
    Anti-COL1A2 antibody (ab84713) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 129 kDa
    Observed band size : 144 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 92 kDa. We are unsure as to the identity of these extra bands.

References for Anti-COL1A2 antibody (ab84713)

ab84713 has not yet been referenced specifically in any publications.

Publishing research using ab84713 ? Please let us know so that we can cite the reference in this datasheet

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Western blot abreview for Anti-COL1A2 antibody

 Inconclusive
Abreviews
Application Western blot
Sample Dog Tissue lysate - whole (kidney, bladder, ureter)
Loading amount 200 µg
Specification kidney, bladder, ureter
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Mar 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"