Overview

  • Product nameAnti-COL1A2 antibody
    See all COL1A2 primary antibodies
  • Description
    Rabbit polyclonal to COL1A2
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 1044 - 1093 (PGSVGPAGPR GPAGPSGPAG KDGRTGHPGT VGPAGIRGPQ GHQGPAGPPG) of Human COL1A2 (NP_000080)

  • Positive control
    • Fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in diseaseEhlers-Danlos syndrome 7B
    Osteogenesis imperfecta 1
    Osteogenesis imperfecta 2
    Ehlers-Danlos syndrome, autosomal recessive, cardiac valvular form
    Osteogenesis imperfecta 3
    Osteogenesis imperfecta 4
    A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
  • DomainThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 2 collagen type I antibody
    • Alpha 2 type I collagen antibody
    • Alpha 2(I) collagen antibody
    • Alpha-2 type I collagen antibody
    • CO1A2_HUMAN antibody
    • COL1A2 antibody
    • Collagen alpha 2(I) chain antibody
    • Collagen alpha-2(I) chain antibody
    • Collagen I alpha 2 polypeptide antibody
    • Collagen of skin tendon and bone alpha 2 chain antibody
    • Collagen type I alpha 2 antibody
    • OI4 antibody
    • Osteogenesis imperfecta type IV antibody
    • Type I procollagen antibody
    see all

Anti-COL1A2 antibody images

  • Anti-COL1A2 antibody (ab84713) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 129 kDa
    Observed band size : 144 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 92 kDa. We are unsure as to the identity of these extra bands.

References for Anti-COL1A2 antibody (ab84713)

ab84713 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Dog Tissue lysate - whole (kidney, bladder, ureter)
Loading amount 200 µg
Specification kidney, bladder, ureter
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Mar 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"