COLO205 whole cell lysate (ab95356)

Overview

  • Product name
    COLO205 whole cell lysate
  • General notes

    Growth Properties: mixed, adherent and suspension
    Morphology: epithelial
    Organ: colon
    Tumor Stage: Dukes' type D
    Disease: colorectal adenocarcinoma
    Derived from metastatic site: ascites
    Cellular Products: carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10)
    Tumorigenic: Yes

    This is a recommended negative control for PD-L1 RabMAb (clone 28-8), Ab205921

  • Tested applications
    Suitable for: WB, SDS-PAGEmore details

Properties

  • Form
    Lyophilised:Add 100 µl distilled water to the sample. Final concentration is 2 mg/ml.
  • Storage instructions
    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituents: 0.1% Bromophenol blue, 2% SDS, 10% Glycerol, 1.54% DTT, 0.79% Tris HCl
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Lysate notes

    This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen. The cells are CSAp negative (CSAp-). The line was derived from tissue from the same patient as COLO 201. The cells are positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.

Applications

Our Abpromise guarantee covers the use of ab95356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
SDS-PAGE Use at an assay dependent concentration.

References

ab95356 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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