Anti-COMP/Cartilage oligomeric matrix protein antibody (ab231977)
Key features and details
- Rabbit polyclonal to COMP/Cartilage oligomeric matrix protein
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-COMP/Cartilage oligomeric matrix protein antibody
See all COMP/Cartilage oligomeric matrix protein primary antibodies -
Description
Rabbit polyclonal to COMP/Cartilage oligomeric matrix protein -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human COMP/Cartilage oligomeric matrix protein aa 100-250. (Expressed in E.coli).
Database link: P49747 -
Positive control
- WB: Recombinant human COMP/Cartilage oligomeric matrix protein; Human and mouse cartilage tissue lysate. IHC-P: Human testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 83 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 83 kDa. |
Target
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Function
May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. -
Tissue specificity
Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect. -
Involvement in disease
Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1) [MIM:132400]. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types.
Defects in COMP are the cause of pseudoachondroplasia (PSACH) [MIM:177170]. PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. -
Sequence similarities
Belongs to the thrombospondin family.
Contains 4 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 8 TSP type-3 repeats. -
Developmental stage
Present during the earliest stages of limb maturation and is later found in regions where the joints develop. -
Domain
The cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response.
The TSP C-terminal domain mediates interaction with FN1 and ACAN. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 281088 Cow
- Entrez Gene: 1311 Human
- Entrez Gene: 12845 Mouse
- Entrez Gene: 25304 Rat
- Omim: 600310 Human
- SwissProt: P35445 Cow
- SwissProt: P49747 Human
- SwissProt: Q9R0G6 Mouse
see all -
Alternative names
- cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple) antibody
- Cartilage oligomeric matrix protein antibody
- Cartilage oligomeric matrix protein precursor antibody
see all
Images
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Formalin-fixed, paraffin-embedded human testis tissue stained for COMP/Cartilage oligomeric matrix protein using ab231977 at 20 µg/mLin immunohistochemical analysis. DAB staining.
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Anti-COMP/Cartilage oligomeric matrix protein antibody (ab231977) at 3 µg/ml + Recombinant human COMP/Cartilage oligomeric matrix protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 83 kDa -
All lanes : Anti-COMP/Cartilage oligomeric matrix protein antibody (ab231977) at 3 µg/ml
Lane 1 : Human cartilage tissue lysate
Lane 2 : Mouse cartilage tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 83 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab231977 has been referenced in 13 publications.
- Boneski PK et al. Abcc6 Null Mice-a Model for Mineralization Disorder PXE Shows Vertebral Osteopenia Without Enhanced Intervertebral Disc Calcification With Aging. Front Cell Dev Biol 10:823249 (2022). PubMed: 35186933
- Ottone OK et al. The cGAS-STING Pathway Affects Vertebral Bone but Does Not Promote Intervertebral Disc Cell Senescence or Degeneration. Front Immunol 13:882407 (2022). PubMed: 35769461
- Armaka M et al. Single-cell multimodal analysis identifies common regulatory programs in synovial fibroblasts of rheumatoid arthritis patients and modeled TNF-driven arthritis. Genome Med 14:78 (2022). PubMed: 35879783
- Vassallo V et al. Unsulfated biotechnological chondroitin by itself as well as in combination with high molecular weight hyaluronan improves the inflammation profile in osteoarthritis in vitro model. J Cell Biochem 122:1021-36 (2021). PubMed: 34056757
- Novais EJ et al. Long-term treatment with senolytic drugs Dasatinib and Quercetin ameliorates age-dependent intervertebral disc degeneration in mice. Nat Commun 12:5213 (2021). PubMed: 34480023