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Western blot - COMT antibody (ab36144)
(enlarge)
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Product Name
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COMT antibody
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See all COMT antibodies (5)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to COMT
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Immunogen
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Synthetic peptide: GDTKEQRILNHVLQC, corresponding to amino acids 52-65 of Human COMT
BLAST 'GDTKEQRILNHVLQC' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Dog
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: 1/4000.
WB: Use at a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa).
(30.0kDa according to NP_000745.1 and of 24.5kDa according to NP_009294.1)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Testis lysate
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Cellular localization
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Single-pass type II membrane protein; extracellular side.
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Research areas
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Signal Transduction >> Metabolism >> Drug metabolism
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
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Relevance
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Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this COMT antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for COMT antibody images, references, products related to ab36144 and other tools.
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COMT antibody images:
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 Western blot - COMT antibody (ab36144)
COMT antibody (ab36144) at 0.2 µg/ml + Human testis lysates at 35 µg
developed using the ECL technique
Predicted band size : 30 kDa
Observed band size : 26,30 kDa (why is the actual band size different from the predicted?)
Primary antibody incubated for 1 hour.
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References for COMT antibody (ab36144)
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This product has been used in:
Glen A et al. iTRAQ-Facilitated Proteomic Analysis of Human Prostate Cancer Cells Identifies Proteins Associated with Progression. J Proteome Res 7:897-907 (2008). WB; Human. PubMed: 18232632
If you publish research using ab36144 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to COMT
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COMT antibody - more information
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Customer reviews (feedback) regarding COMT antibody |
Customer FAQs regarding COMT antibody |
Protocols for COMT antibody |
Price and availability of products related to COMT antibody |
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COMT antibody - related products:
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COMT antibody - other tools:
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Contact Abcam with a Technical Enquiry about COMT antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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