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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Overview
Product name
Anti-COMT antibody
See all COMT products (10) ...
Description
Goat polyclonal to COMT
Specificity
This antibody is expected to recognise both reported isoforms.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Horse, Dog
Immunogen
Synthetic peptide: GDTKEQRILNHVLQC, corresponding to amino acids 52-65 of Human COMT
GDTKEQRILN HVLQC
Positive control
Human testis lysate .
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Drug metabolism
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
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Western blot - COMT antibody (ab36144)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.3 - 1 µg/ml.(Approx 26kDa plus 29kDa bands observed in Human Testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and of 24.5kDa according to NP_009294.1).)
Target
Function
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Tissue specificity
Brain, liver, placenta, lymphocytes and erythrocytes.
Sequence similarities
Belongs to the mammalian catechol-O-methyltransferase family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Cytoplasm and Cell membrane.
Target information above from: UniProt accessionP21964
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Catechol O methyltransferase antibody
- Catechol O-methyltransferase antibody
- COMT antibody
see all
Database links
- Entrez Gene: 445450 Dog
- Entrez Gene: 1312 Human
- Entrez Gene: 12846 Mouse
- Entrez Gene: 24267 Rat
- Omim: 116790 Human
- SwissProt: P21964 Human
- SwissProt: O88587 Mouse
- SwissProt: P22734 Rat
see all
Anti-COMT antibody images:
Western blot - COMT antibody (ab36144)
Anti-COMT antibody (ab36144) at 0.3 µg/ml + Human testis lysates in RIPA buffer at 35 µg
Observed band size : 26,29 kDa (why is the actual band size different from the predicted?)
Primary antibody incubated for 1 hour.
References for Anti-COMT antibody (ab36144)
This product has been referenced in:
- Glen Aet al. iTRAQ-Facilitated Proteomic Analysis of Human Prostate Cancer Cells Identifies Proteins Associated with Progression. J Proteome Res 7:897-907 (2008). WB; Human.Read more (PubMed: 18232632) »
See 1 publication for this product
Publishing research using ab36144? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"