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Western blot - COMT antibody (ab51984)
(enlarge)
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Product Name
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COMT antibody
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See all COMT antibodies (5)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to COMT
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Immunogen
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Synthetic peptide: C-QDIIPQLKKKYDVD, corresponding to internal sequence amino acids 170-183 of Human COMT
BLAST 'C-QDIIPQLKKKYDVD' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Dog, Hrs, Pig
(Due to sequence homology)
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Specificity
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This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1).
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Testis lysates
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Cellular localization
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Single-pass type II membrane protein; extracellular side.
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Research areas
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Signal Transduction >> Metabolism >> Drug metabolism
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
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Relevance
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Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this COMT antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for COMT antibody images, references, products related to ab51984 and other tools.
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COMT antibody images:
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 Western blot - COMT antibody (ab51984)
COMT antibody (ab51984) at 0.03 µg/ml + Human Testis lysate in RIPA buffer at 35 µg
Predicted band size : 30 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
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Search PubMed (MEDLINE) for references to COMT
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COMT antibody - more information
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Customer reviews (feedback) regarding COMT antibody |
Customer FAQs regarding COMT antibody |
Protocols for COMT antibody |
Price and availability of products related to COMT antibody |
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COMT antibody - related products:
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COMT antibody - other tools:
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Contact Abcam with a Technical Enquiry about COMT antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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