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Western blot - COMT antibody (ab66073)
(enlarge)
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Product Name
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COMT antibody
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See all COMT antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to COMT
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Immunogen
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Recombinant full length protein (Human) with tag.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A431 cell lysate.
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Cellular localization
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Single-pass type II membrane protein; extracellular side.
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Research areas
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Signal Transduction >> Metabolism >> Drug metabolism
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
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Relevance
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Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2b
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this COMT antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for COMT antibody images, references, products related to ab66073 and other tools.
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COMT antibody images:
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 Western blot - COMT antibody (ab66073)
COMT antibody (ab66073) at 1 µg/ml + A431 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 30 kDa
Observed band size : 30 kDa
Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to COMT
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COMT antibody - more information
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Customer reviews (feedback) regarding COMT antibody |
Customer FAQs regarding COMT antibody |
Protocols for COMT antibody |
Price and availability of products related to COMT antibody |
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COMT antibody - related products:
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COMT antibody - other tools:
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Contact Abcam with a Technical Enquiry about COMT antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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