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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Overview
Product name
Anti-COMT antibody
See all COMT products (10) ...
Description
Mouse monoclonal to COMT
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human) with tag.
Positive control
A431 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2b
Research areas
Signal Transduction >> Metabolism >> Drug metabolism
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
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Western blot - COMT antibody (ab66073)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
Target
Function
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Tissue specificity
Brain, liver, placenta, lymphocytes and erythrocytes.
Sequence similarities
Belongs to the mammalian catechol-O-methyltransferase family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Cytoplasm and Cell membrane.
Target information above from: UniProt accessionP21964
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Catechol O methyltransferase antibody
- Catechol O-methyltransferase antibody
- COMT antibody
see all
Database links
- Entrez Gene: 1312 Human
- Omim: 116790 Human
- SwissProt: P21964 Human
- Unigene: 370408 Human
- Unigene: 704514 Human
- Unigene: 713616 Human
Anti-COMT antibody images:
Western blot - COMT antibody (ab66073)
Anti-COMT antibody (ab66073) at 1 µg/ml + A431 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 30 kDa
Observed band size : 30 kDa
Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-COMT antibody (ab66073)
ab66073 has not yet been referenced specifically in any publications.
Publishing research using ab66073? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"