COP 293T Transfected Lysate - (positive control) (ab94203)

Overview

  • Product nameCOP 293T Transfected Lysate - (positive control)
  • General notesab94203 is a 293T cell transfected lysate in which Human COP has been transiently over-expressed using a pCMV-COP plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research Areas
  • BackgroundFunction: Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2. Tissue specificity: Widely expressed. Expressed at higher level in placenta, spleen, lymph node and bone marrow. Weakly or not expressed in thymus. Similarity: Contains 1 CARD domain.

Applications

Our Abpromise guarantee covers the use of ab94203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • COP 293T Transfected Lysate - (positive control) images

    • ab94203 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-COP antibody (ab57714) at 1/500 dilution

      Lane 1 : COP 293T Transfected Lysate - (positive control) (ab94203)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for COP 293T Transfected Lysate - (positive control) (ab94203)

    ab94203 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"