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Western blot - COP1 antibody (ab56400)
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Immunocytochemistry/ Immunofluorescence - COP1 antibody (ab56400)
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Product Name
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COP1 antibody
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See all COP1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to COP1
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Immunogen
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Recombinant fragment: CLRSFKGHIN EKNFVGLASN GDYIACGSEN NSLYLYYKGL SKTLLTFKFD TVKSVLDKDR KEDDTNEFVS AVCWRALPDG ESNVLIAANS QGTIKVLELV , corresponding to amino acids 632-732 of Human COP1
BLAST 'CLRSFKGHIN EKNFVGLASN GDYIACGSEN NSLYLYYKGL SKTLLTFKFD TVKSVLDKDR KEDDTNEFVS AVCWRALPDG ESNVLIAANS QGTIKVLELV ' with  or 
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Reacts with
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Hu, Ms
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 80 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear and cytoplasmic. In the nucleus, it forms nuclear speckles.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Relevance
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COP1 is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. It is directly involved in p53 ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. It ubiquitinates p53 independently of MDM2 or RCHY1. It probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, where the ubiquitin ligase activity is mediated by RBX1.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.400 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this COP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for COP1 antibody images, references, products related to ab56400 and other tools.
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COP1 antibody images:
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 Western blot - COP1 antibody (ab56400)
Predicted band size : 80 kDa
COP1 antibody (ab56400) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
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Immunocytochemistry/ Immunofluorescence - COP1 antibody (ab56400)
Immunofluorescence of ab56400 on NIH/3T3 cell [antibody concentration 10 ug/ml].
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Search PubMed (MEDLINE) for references to COP1
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COP1 antibody - more information
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Customer reviews (feedback) regarding COP1 antibody |
Customer FAQs regarding COP1 antibody |
Protocols for COP1 antibody |
Price and availability of products related to COP1 antibody |
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COP1 antibody - related products:
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COP1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about COP1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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