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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - COP1 antibody (ab70889)
All lanes : Anti-COP1 antibody (ab70889) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 80 kDa
Observed band size : 80 kDa
Exposure time : 3 minutes
Immunoprecipitation - COP1 antibody (ab70889)
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70889 at 1 µg/ml for WB, subsequent to IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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