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Western blot - COP1 antibody (ab70889)
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Immunoprecipitation - COP1 antibody (ab70889)
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Product Name
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COP1 antibody
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See all COP1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to COP1
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Immunogen
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A region between residue 1 and 50 of human COP1. (NP_071902.2)
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at a concentration of 2 - 5 µg/ml.
WB: 1/2000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa, 293T and NIH3T3 whole cell lysates.
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Cellular localization
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Nuclear and cytoplasmic. In the nucleus, it forms nuclear speckles.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Relevance
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COP1 is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. It is directly involved in p53 ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. It ubiquitinates p53 independently of MDM2 or RCHY1. It probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, where the ubiquitin ligase activity is mediated by RBX1.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this COP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for COP1 antibody images, references, products related to ab70889 and other tools.
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COP1 antibody images:
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 Western blot - COP1 antibody (ab70889)
All lanes : COP1 antibody (ab70889) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 80 kDa
Observed band size : 80 kDa
Exposure time : 3 minutes
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Immunoprecipitation - COP1 antibody (ab70889)
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70889 at 1 µg/ml for WB, subsequent to IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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Search PubMed (MEDLINE) for references to COP1
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COP1 antibody - more information
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Customer reviews (feedback) regarding COP1 antibody |
Customer FAQs regarding COP1 antibody |
Protocols for COP1 antibody |
Price and availability of products related to COP1 antibody |
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COP1 antibody - related products:
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COP1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about COP1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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