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Overview
Product name
Anti-COP1 antibody
See all COP1 products (6) ...
Description
Rabbit polyclonal to COP1
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A region between residues 300 and 350 of human COP1. (NP_071902.2)
Positive control
HeLa whole cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Immunoprecipitation - COP1 antibody (ab70891)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IP
Application notes
IP: Use at a concentration of 2 - 5 µg/ml.
Is unsuitable for WB. (Use ab70889).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
Tissue specificity
Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the COP1 family.
Contains 1 RING-type zinc finger.
Contains 7 WD repeats.
Domain
The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).
Cellular localization
Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.
Target information above from: UniProt accessionQ8NHY2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Constitutive photomorphogenesis protein 1 homolog antibody
- COP antibody
- E3 ubiquitin-protein ligase RFWD2 antibody
see all
Database links
- Entrez Gene: 64326 Human
- Entrez Gene: 26374 Mouse
- SwissProt: Q8NHY2 Human
- SwissProt: Q9R1A8 Mouse
- Unigene: 523744 Human
- Unigene: 328135 Mouse
Anti-COP1 antibody images:
Immunoprecipitation - COP1 antibody (ab70891)
HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
Antibodies:
Lane 1: ab70889 at 3µg/ml for IP.
Lane 2: ab70890 at 3µg/ml for IP.
Lane 3: ab70891 at 3µg/ml for IP.
Lane 4: IgG control
Subsequent Western Blotting was carried out using ab70889 at 1µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
References for Anti-COP1 antibody (ab70891)
ab70891 has not yet been referenced specifically in any publications.
Publishing research using ab70891? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"