COS7 (African Green Monkey SV40-transf'd kidney fibroblast cell line) Nuclear Lysate (ab14643)

Overview

  • Product nameCOS7 (African Green Monkey SV40-transf'd kidney fibroblast cell line) Nuclear LysateSee all COS7 lysates ...
  • General notesWe recommend aliquoting the extracts into single use fractions and then storing.
  • Tested applicationsWB, EMSA more details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • BackgroundThese fibroblast-like cells grow in monolayers and are possible hosts for the propagation of pure populations of recombinant SV40 virus and contain T antigen of SV 40 virus. They are useful in virus replication studies and transfection studies.

Applications

Our Abpromise guarantee covers the use of ab14643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
EMSA
  • Application notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for COS7 (African Green Monkey SV40-transf'd kidney fibroblast cell line) Nuclear Lysate (ab14643)

    ab14643 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"