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Western blot - COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)
![Western blot - COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)](/ps/datasheet/images/ab14744_1.jpg)
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Immunocytochemistry/ Immunofluorescence - COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)
![Immunocytochemistry/ Immunofluorescence - COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)](/ps/datasheet/images/ab14744_7.jpg)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)
![Immunohistochemistry (PFA perfusion fixed frozen sections) - COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)](/ps/datasheet/images/14/ab14744/COX-IV-Primary-antibodies-ab14744-7.jpg)
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Product Name
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COX IV antibody [20E8] - Mitochondrial Loading Control
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See all COX IV - Loading Control antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [20E8] to COX IV - Mitochondrial Loading Control
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Immunogen
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Purified mitochondrial Complex IV (Cow).
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Hm
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at a concentration of 5 µg/ml.
ICC/IF: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution (PMID 18698340).
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 17 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human heart mitochondria.
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Cellular localization
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Mitochondrial inner membrane
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Isotype/Loading Controls >> Loading Controls >> COX IV - Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Relevance
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Cytochrome C Oxidase (COX), which is located in the inner mitochondrial membrane, is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced CYTOCHROME C and donates them to molecular oxygen, which is then reduced to water. It is composed of CYTOCHROME A and CYTOCHROME B, two copper atoms, and 13 different protein subunits, three of which are encoded by the mitochondrial DNA and ten others by nuclear DNA (mammals).
This antibody makes an effective loading control for mitochondria. COXIV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COXIV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Rabbit polyclonal to COX IV antibody (ab16056).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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20E8
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this COX IV antibody [20E8] - Mitochondrial Loading Control is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for COX IV antibody [20E8] - Mitochondrial Loading Control images, references, products related to ab14744 and other tools.
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COX IV antibody [20E8] - Mitochondrial Loading Control images:
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 Western blot - COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)
All lanes : COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744) at 1/5000 dilution
Lane 1 : Cytoplasmic fraction mouse NIH/3T3 cell lysate
Lane 2 : Nuclear fraction mouse NIH/3T3 cell lysate
Secondary
HRP conjugated goat anti-mouse antibody
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 16 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
This image is courtesy of an Abreview submitted by Camilla Skjerpen on 4 July 2005.
See Abreview
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Immunocytochemistry/ Immunofluorescence - COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)
ab14744 at 1/100 staining MCF-10A cells (human mammary epithelial cell line) by ICC/IF. The cells were methanol/acetone fixed at -20C for 5 minutes, blocked with BSA and then incubated with the antibody for 16 hours. The positive tissue was colocalised with a mitotracker (mitotrackers are a series of patented mt-selective stains that are concentrated by active mt and well retained during cell fixation). An Alexa-Fluor ® 488 conjugated goat anti-mouse antibody was used as the secondary. The image shows COXIV staining in green and DAPI staining in blue. This image is courtesy of an anonymous Abreview
See Abreview
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Immunohistochemistry (PFA perfusion fixed frozen sections) - COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)
ab14744 staining COX IV in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Rats were anesthetized and intracardially perfused with 500 ml of normal saline at room temperature, followed by 500 ml of ice-cold, freshly made 4% paraformaldehyde in phosphate buffer (PB, 0.1 M, pH 7.4). A Cy3 conjugated anti mouse antibody was used as secondary. Image from Hashimoto T. et. al., PLoS ONE. 2008; 3(8): e2915 (Fig. 1B)
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References for COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)
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This product has been used in: (two most recent references)
Beirowski B et al. Non-nuclear Wld(S) determines its neuroprotective efficacy for axons and synapses in vivo. J Neurosci 29:653-68 (2009). PubMed: 19158292
Mou Z et al. The armadillo repeat-containing protein, ARMCX3, physically and functionally interacts with the developmental regulatory factor Sox10. J Biol Chem 284:13629-40 (2009). WB, ICC/IF; Mouse. PubMed: 19304657
See all 36 publication references for this product.
If you publish research using ab14744 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to COX IV
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COX IV antibody [20E8] - Mitochondrial Loading Control - more information
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COX IV antibody [20E8] - Mitochondrial Loading Control - related products:
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COX IV antibody [20E8] - Mitochondrial Loading Control - other tools:
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Contact Abcam with a Technical Enquiry about COX IV antibody [20E8] - Mitochondrial Loading Control |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)](/index.html?datasheet=14744#ab14744_1.jpg)
![Immunocytochemistry/ Immunofluorescence - COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)](/index.html?datasheet=14744#ab14744_7.jpg)
![Immunohistochemistry (PFA perfusion fixed frozen sections) - COX IV antibody [20E8] - Mitochondrial Loading Control (ab14744)](/index.html?datasheet=14744#COX-IV-Primary-antibodies-ab14744-7.jpg)
