Anti-COX IV antibody [20E8C12] - Mitochondrial Loading Control (ab14744)

I am getting high background in ICC staining 3T3 fibroblasts with 1:100 of primary; block with BSA and Triton X-100 for 15 min; secondary is Alexa 488 conjugate;

ANSWER:

Thank you for your enquiry.

Here is the link we discussed, to our ICC protocol:
http://www.abcam.com/index.html?pageconfig=resource&rid=11417

I hope this is helpful. Please contact me again if you have any further questions.

Would like to stain mitochondria in NIH3T3 cells.

ANSWER:

Thank you for your call today and for your enquiry.

As we discussed, I would recommend either ab14744 or ab15895 for immunostaining of mitochondria in mouse cells-

http://www.abcam.com/COX-IV-antibody-20E8C12-Mitochondrial-Loading-Control-ab14744.html

http://www.abcam.com/VDAC1-Porin-antibody-Mitochondrial-Loading-Control-ab15895.html

We additionally have a CytoPainter mitochondrial dye, ab112143, that would be suitable to visualize mitochondria in live cells-

http://www.abcam.com/CytoPainter-Mitochondrial-Staining-Kit-Green-Fluorescence-ab112143.html

I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

Kindly advise if ab110261, ab 63947 will cross react with mouse samples (for WB).

ANSWER:

Thank you for contacting us.
Unfortunately, ab110261 has been tested and found to be unsuitable for use on mouse samples, and ab63947 was raised against a very short immunogen that shows only 69% homology with mouse. We would not expect either of these antibodies to give good results for mouse samples.
If you are looking for an antibody to COX subunit IV, ab14744 works well in a number of applications and species, including mouse and WB. I hope this helps, please let me know if you need any additional information or assistance.

we purchased twice a mouse monoclonal antibody to COX IV (ab14744) that we used for western blotting. The first lot of the ab (GR46848-8, ORDER xxx) worked very well but the new lot we got last time (GR63356-1, ORDER xxx) didn't work at all. We followed the same protocol, and we used the same samples, but we didn't have any signal in our blotting for COX IV ( Please see the figure attached). We tried several times, also with other samples, but we never got any band for COX. The storage for both antibody was the same, at +4C as recommended in the datasheet. At this point, we believe that this new lot you sent us doesn't work properly.

Therefore, we would really appreciate if you could send us another lot of antibody to replace this bad one.

ANSWER:

Thank you for your email and for sending the data.
I am very sorry to hear that the recent lot did not perform as expected.
The details you provided enable us to closely monitor the quality of our products.
I am sorry this recent product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with a new lot number.
I hope the new vial will work as it should. You should receive it tomorrow.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Dear Sir/Madam,

I was trying to get a datasheet for the product MS602 but I failed on your homepage and the one of Mitoscience.

Can you please send me the datasheet.

In fact, what I would need is an OXphos/PHD human AK kit for IHC and WB, if possible!
Kind regards

ANSWER:

Thank you for your enquiry. It has taken some time to confirm all the details for you, and I appreciate your patience.

I am sorry to confirm that MS602 has been discontinued and we do not have anymore in stock.

Although that product is not available any longer, there is ab110411, Total OXPHOS Human WB Antibody Cocktail and ab110416, Pyruvate dehydrogenase (PDH) WB Antibody Cocktail, however as the name suggests they have only been tested in WB, not IHC-P.

If you would like to considering trying to recreate the original antibody cocktail,I have listed the individual components are below.

Complex I 17G3D9E12 ab110243
http://www.abcam.com/index.html?datasheet=110243 (or use the following: http://www.abcam.com/index.html?datasheet=110243).

Complex II ab14714
http://www.abcam.com/index.html?datasheet=14714 (or use the following: http://www.abcam.com/index.html?datasheet=14714).

Complex III 13G12AF12BB11 ab14745
http://www.abcam.com/index.html?datasheet=14745 (or use the following: http://www.abcam.com/index.html?datasheet=14745).

Complex IV 1D6E1A8 ab14705
http://www.abcam.com/index.html?datasheet=14705 (or use the following: http://www.abcam.com/index.html?datasheet=14705).

Complex V 4C11C10D12 ab110276
http://www.abcam.com/index.html?datasheet=110276 (or use the following: http://www.abcam.com/index.html?datasheet=110276).

PDH 9H9AF5 ab110330
http://www.abcam.com/index.html?datasheet=110330 (or use the following: http://www.abcam.com/index.html?datasheet=110330).

As there are several components, we would be able to offer you a discount if you wish to purchase all of these products together. I am forwarding this to our bulk orders team who will be able to provide a quote for you.

Otherwise, I am sorry there is nothing else to suggest that would be a suitable replacement on this occasion.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.