Overview
- Product nameAnti-COX IV antibody [6B3]See all COX IV primary antibodies ...
- DescriptionMouse monoclonal [6B3] to COX IV
- Tested applicationsELISA, Flow Cyt, ICC, WB more details
- Species reactivityReacts with: Mouse, Rat, Human, Monkey
- Immunogen
Purified recombinant fragment of Human COX IV expressed in E. Coli
- Positive controlHEK293, A549 and PC12 cell lysates PANC-1 cells K562 cells
Properties
- FormLiquid
- Storage instructionsStore at 4°C. Aliquot and store at -20°C long-term.
- Storage bufferPreservative: 0.03% Sodium Azide
Constituents: Ascites -
Concentration information loading... - PurityAscites
- Clonality Monoclonal
- Clone number6B3
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab105915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/10000. |
| Flow Cyt | Flow Cyt: Use at an assay dependent dilution. |
| ICC | ICC: 1/200 - 1/1000. |
| WB | WB: 1/500 - 1/2000. Predicted molecular weight: 20 kDa. |
Target
- FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
- Tissue specificityUbiquitous.
- Sequence similaritiesBelongs to the cytochrome c oxidase IV family.
- Cellular localizationMitochondrion inner membrane.
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Database links
- Entrez Gene: 1327 Human
- Entrez Gene: 1327 Human
- Entrez Gene: 12857 Mouse
- Entrez Gene: 29445 Rat
- Omim: 123864 Human
- SwissProt: P13073 Human
- SwissProt: P13073 Human
- SwissProt: P19783 Mouse
- SwissProt: P10888 Rat
- Unigene: 433419 Human
- Unigene: 386758 Mouse
- Unigene: 2528 Rat
see all
Target information above from: UniProt accession
P13073
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AL024441 antibodyCOX 4 antibodyCOX IV 1 antibody
- COX IV antibodyCOX IV-1 antibodyCox4 antibodyCOX41_HUMAN antibodyCox4a antibodyCOX4B antibodyCOX4I1 antibodyCOX4I2 antibodyCOX4L2 antibodyCOXIV antibodyCytochrome c oxidase polypeptide IV antibodyCytochrome c oxidase subunit 4 isoform 1 antibodyCytochrome c oxidase subunit 4 isoform 1 mitochondrial antibodyCytochrome c oxidase subunit 4 isoform 1, mitochondrial antibodyCytochrome C Oxidase subunit IV antibodyCytochrome c oxidase subunit IV isoform 1 antibodyCytochrome c oxidase subunit IV isoform 2 (lung) antibodyCytochrome c oxydase subunit 4 antibodydJ857M17.2 antibodyMGC105470 antibodyMGC72016 antibodymitochondrial antibody
see all
Anti-COX IV antibody [6B3] images
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![Western blot - COX IV antibody [6B3] (ab105915)](http://a.abcam.com/ps/datasheet/images/105/ab105915/COX-IV-Primary-antibodies-ab105915-1.jpg)
Western blot - COX IV antibody [6B3] (ab105915)All lanes : Anti-COX IV antibody [6B3] (ab105915) at 1/500 dilution
Lane 1 : HEK293 cell lysate.
Lane 2 : A549 cell lysate.
Lane 3 : PC12 cell lysate.
Predicted band size : 20 kDa -
![Immunocytochemistry/ Immunofluorescence - COX IV antibody [6B3] (ab105915)](http://a.abcam.com/ps/datasheet/images/105/ab105915/COX-IV-Primary-antibodies-ab105915-2.jpg)
Immunocytochemistry/ Immunofluorescence - COX IV antibody [6B3] (ab105915)Immunofluorescence analysis of PANC-1 cells using ab105915 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. -
![Flow Cytometry - COX IV antibody [6B3] (ab105915)](http://a.abcam.com/ps/datasheet/images/105/ab105915/COX-IV-Primary-antibodies-ab105915-3.jpg)
Flow Cytometry - COX IV antibody [6B3] (ab105915)Flow cytometric analysis of K562 cells using ab105915 (blue) and negative control (red).
References for Anti-COX IV antibody [6B3] (ab105915)
ab105915 has not yet been referenced specifically in any publications.
