Anti-COX IV antibody [6B3] (ab105915)

Overview

  • Product nameAnti-COX IV antibody [6B3]See all COX IV primary antibodies ...
  • Description
    Mouse monoclonal [6B3] to COX IV
  • Tested applicationsELISA, Flow Cyt, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Purified recombinant fragment of Human COX IV expressed in E. Coli

  • Positive control
    • HEK293, A549 and PC12 cell lysates PANC-1 cells K562 cells

Applications

Our Abpromise guarantee covers the use of ab105915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
Flow Cyt Use at an assay dependent dilution.
ICC 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 20 kDa.

Target

  • FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the cytochrome c oxidase IV family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL024441 antibody
    • COX 4 antibody
    • COX IV 1 antibody
    • COX IV antibody
    • COX IV-1 antibody
    • Cox4 antibody
    • COX41_HUMAN antibody
    • Cox4a antibody
    • COX4B antibody
    • COX4I1 antibody
    • COX4I2 antibody
    • COX4L2 antibody
    • COXIV antibody
    • Cytochrome c oxidase polypeptide IV antibody
    • Cytochrome c oxidase subunit 4 isoform 1 antibody
    • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
    • Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
    • Cytochrome C Oxidase subunit IV antibody
    • Cytochrome c oxidase subunit IV isoform 1 antibody
    • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxydase subunit 4 antibody
    • dJ857M17.2 antibody
    • MGC105470 antibody
    • MGC72016 antibody
    • mitochondrial antibody
    see all

Anti-COX IV antibody [6B3] images

  • All lanes : Anti-COX IV antibody [6B3] (ab105915) at 1/500 dilution

    Lane 1 : HEK293 cell lysate.
    Lane 2 : A549 cell lysate.
    Lane 3 : PC12 cell lysate.


    Predicted band size : 20 kDa
  • Immunofluorescence analysis of PANC-1 cells using ab105915 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of K562 cells using ab105915 (blue) and negative control (red).

References for Anti-COX IV antibody [6B3] (ab105915)

ab105915 has not yet been referenced specifically in any publications.

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for your reply.

I would recommend ab14744 as a suitable replacement:

http://www.abcam.com/COX-IV-antibody-20E8C12-Mitochondrial-Loading-Control-ab14744.html



If you would like to receive this antibody a...

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Thank you for contacting Abcam.

I am very sorry that the suggestions provided did not improve the performance of this product for you and wish to thank you for the time which you have put into troubleshooting this.

We would of cou...

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Thank you for this additional information.

I would like to offer additional suggestions based on this new information which may help you achieve good results using ab105915. When performing western blot on mitochondrial proteins there are a ...

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Thank you for taking time to provide these details. I am sorry to hear that this antibody is not providing satisfactory results.

The details will enable us to investigate this case and will provide us with vital information for monitoring ...

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Thank you for contacting us.

I am sorry to hear that you feel the product is not performing as it should and would like to help you with this problem. Often, small changes in a protocol may remedy many issues. Two of the most common causes...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (retina)
Specification retina
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"