Anti-COX IV antibody (ab66739)

Overview

• Product nameAnti-COX IV antibodySee all COX IV primary antibodies ...
• Description
  Rabbit polyclonal to COX IV
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Chimpanzee
• Immunogen

  A region within synthetic peptide: AISTSVCVRA HESVVKSEDF SLPAYMDRRD HPLPEVAHVK HLSASQKALK, corresponding to amino acids 14-63 of Human COX IV

  Run BLAST with Run BLAST with
• Positive controlHepG2 cell lysate. Human heart tissue. Human kidney tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: mcf-7.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Oxidative phosphorylation
  • Complex IV

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Isotype/Loading Controls
  • Loading Controls
  • COX IV - Mitochondrial

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

Applications

Target

• FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the cytochrome c oxidase IV family.
• Cellular localizationMitochondrion inner membrane.

Target information above from: UniProt accession P13073 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 281090 Cow
  • Entrez Gene: 1327 Human
  • Entrez Gene: 1327 Human
  • Entrez Gene: 29445 Rat
  • Omim: 123864 Human
  • SwissProt: P00423 Cow
  • SwissProt: P13073 Human
  • SwissProt: P13073 Human

  • SwissProt: P10888 Rat
  • Unigene: 433419 Human
  • Unigene: 2528 Rat

  see all

• Alternative names
  AL024441 antibodyCOX 4 antibodyCOX IV 1 antibody

  COX IV antibodyCOX IV-1 antibodyCox4 antibodyCOX41_HUMAN antibodyCox4a antibodyCOX4B antibodyCOX4I1 antibodyCOX4I2 antibodyCOX4L2 antibodyCOXIV antibodyCytochrome c oxidase polypeptide IV antibodyCytochrome c oxidase subunit 4 isoform 1 mitochondrial antibodyCytochrome c oxidase subunit 4 isoform 1, mitochondrial antibodyCytochrome C Oxidase subunit IV antibodyCytochrome c oxidase subunit IV isoform 1 antibodyCytochrome c oxidase subunit IV isoform 2 (lung) antibodyCytochrome c oxydase subunit 4 antibodydJ857M17.2 antibodyMGC105470 antibodyMGC72016 antibody

  see all

Anti-COX IV antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody (ab66739)

  ICC/IF image of ab66739 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab66739 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Western blot - COX IV antibody (ab66739)
  Anti-COX IV antibody (ab66739) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 20 kDa
  Observed band size : <21 kDa (why is the actual band size different from the predicted?)
  Antibody diltued in 5% skim milk / PBS buffer. Gel concentration 12%.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX IV antibody (ab66739)
  ab66739, at 2 µg/ml, staining COX IV in Human heart tissue by immunohistochemistry on paraffin-embedded tissue. The arrows indicate positively stained myocardial cells.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX IV antibody (ab66739)
  ab66739, at 2 µg/ml, staining COX IV in human kidney tissue by immunohistochemistry on paraffin-embedded tissue. Positively stained cells are epithelial cells of the renal tubule.
• 

  Western blot - Anti-COX IV antibody (ab66739)
  All lanes : Anti-COX IV antibody (ab66739)
  
  Lane 1 : Lysate prepared from human liver
  Lane 2 : Lysate prepared from rat liver
  Lane 3 : Lysate prepared from wild type mouse liver
  Lane 4 : Lysate prepared from AMPKa1+2-/- mouse liver
  Lane 5 : Lysate prepared from human muscle
  Lane 6 : Lysate prepared from rat muscle
  Lane 7 : Lysate prepared from mouse muscle
  
  
  Predicted band size : 20 kDa
  A small piece (~30mg) of every organ was homogenized in a 6:1 (v/w) ratio of ice-cold buffer containing: 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (pH 7.6), 50 mM sodium fluoride, 50 mM potassium chloride, 5 mM sodium pyrophosphate, 1 mM ethylenediaminetetraacetic acid, 1 mM ethylene glycol tetraacetic acid, 5 mM ß-glycerophosphate, 1 mM sodium vanadate, 1 mM dithiothreitol, 1% nonyl phenoxypolyethoxylethanol (Tergitol-type NP40) and protease inhibitors cocktail. Homogenates were centrifuged (13,200 rpm; 15 min, 4°C) and the protein content of the supernatant was determined using a bicinchoninic acid (BCA) protein assay kit.
  
  Proteins (30 µg) were separated by either 12.5 or 10% SDS-PAGE followed by transfer to a polyvinylidene fluoride transfer membrane. Membranes were blocked for 1 h at room temperature in tris-buffered saline tween-20 buffer with 5% non-fat dry milk followed by an overnight incubation with antibodies. Blots were then incubated with horseradish