Mouse, Rat, Sheep, Cow, Human, Xenopus laevis, Monkey, African green monkey, Chinese hamster, Drosophila C virus Predicted to work with:
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human COX IV aa 150 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. (Peptide available as ab16381)
WB: Jurkat and HepG2 whole cell lysates and human skeletal muscle, mouse skeletal muscle and cow kidney tissue lysates.
This antibody clone is manufactured by Abcam.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
ICC/IF image of ab33985 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33985, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Overlay histogram showing HeLa cells stained with ab33985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33985, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)This image is courtesy of an Abreview submitted by Dr Anne-Lore Schlaitz
All lanes : Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution
Lane 1 : Crude extract prepared from Xenopus laevis egg Lane 2 : Cytosol lysate prepared from Xenopus laevis egg extract Lane 3 : Total membrane lysate prepared from Xenopus laevis egg extract
Lysates/proteins at 15 µg per lane.
Secondary HRP conjugated donkey anti-mouse IgG at 1/4000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 15 kDa Observed band size : 15 kDa
Exposure time : 90 minutes
This image is courtesy of an Abreview submitted by Dr Anne-Lore Schlaitz
Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)This image is courtesy of an anonymous Abreview
ab33985 staining COX IV in human proximal tubular epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 45 minutes at 20°C. ab6785, a FITC-conjugated goat anti-mouse IgG (H+L) polyclonal was used as the secondary antibody (1/1000).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)This image is courtesy of an anonymous Abreview
ab33985 staining COX IV in mouse kidney (tubules) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% trition X-100 and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in sodium citrate buffer pH 6. Samples were incubated with primary antibody (1/200 in PBS) for 9 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. DAPI was used for staining the nucleus.