Immunocytochemistry/ Immunofluorescence abreview for Anti-COX IV [mAbcam33985] antibody

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (leukocytes from bone marrow)
Specification leukocytes from bone marrow
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution 1/250
Incubation time 12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS

Secondary antibody

Name Non-Abcam antibody was used: Alexa Fluor® 594 goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution 1/500

Additional data

Additional Notes Slide was incubated in methanol for 10 min. 2nd antibody were incubated for 30 min at RT. DAPI was used for staining nuclei.

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Submitted Jan 27 2011