Immunocytochemistry/ Immunofluorescence abreview for Anti-COX IV [mAbcam33985] antibody

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Abreviews
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Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (leukocytes from bone marrow)
Specification
leukocytes from bone marrow
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/250
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor® 594 goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500

Additional data

Additional Notes
Slide was incubated in methanol for 10 min. 2nd antibody were incubated for 30 min at RT. DAPI was used for staining nuclei.
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Submitted Jan 27 2011

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