Western blot abreview for Anti-COX IV [mAbcam33985] antibody

Good
Abreviews
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Application
Western blot
Sample
Sheep Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1.5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: blocking agent(milk)

Secondary antibody

Dilution
1/5000

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 15 kDa
Positive control
did not use positve control

Additional data

Additional Notes
1: Marker;
2: Blank;
3 and 4: liver samples sheep - Cox IV
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Submitted Jul 12 2011

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