Western blot abreview for Anti-COX IV [mAbcam33985] antibody

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Sheep Tissue lysate - whole (liver)
Loading amount 30 µg
Specification liver
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1.5% · Temperature: 25°C

Other product details

Dilution 1/1000
Incubation time 1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: blocking agent(milk)

Secondary antibody

Dilution 1/5000


Detection method ECL
Exposure 30 second(s)
Bands Specific: 15 kDa
Positive control did not use positve control

Additional data

Additional Notes 1: Marker;
2: Blank;
3 and 4: liver samples sheep - Cox IV

Abcam user community

Verified customer

Submitted Jul 12 2011