Western blot abreview for Anti-COX IV [mAbcam33985] antibody

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (hek293)
Loading amount
30 µg
Specification
hek293
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Other product details

Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: 5%milk

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ecl
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: about 15 kDa
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Submitted May 14 2013

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