Western blot abreview for Anti-COX IV [mAbcam33985] antibody

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Abreviews
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Application Western blot
Sample Human Cell lysate - whole cell (hek293)
Loading amount 30 µg
Specification hek293
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Other product details

Dilution 1/1000
Incubation time 2 hour(s) and 0 minute(s) · Diluent: 5%milk

Secondary antibody

Name Non-Abcam antibody was used: anti-mouse IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/4000

Detection

Detection method ecl
Exposure 1 minute(s) and 0 second(s)
Bands Specific: about 15 kDa
Username

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Submitted May 14 2013