Western blot abreview for Anti-COX IV [mAbcam33985] antibody

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (fly heart tube)
Specification fly heart tube
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Other product details

Incubation time 2 hour(s) and 0 minute(s) · Diluent: 5%milk
Dilution 1/1000

Secondary antibody

Name Non-Abcam antibody was used: anti-mouse IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/4000


Detection method ecl
Bands Specific: around 15 kDa
Exposure 1 minute(s) and 0 second(s)

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Submitted Jul 02 2013