Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-COX IV [mAbcam33985] antibody

Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Mouse Tissue sections (mouse heart)
Specification
mouse heart
Permeabilization
Yes - 1% triton x-100
Fixative
Paraformaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Diluent: 5% goat serum
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse IgG-alexa488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400
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Submitted Jul 02 2013

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