Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-COX IV [mAbcam33985] antibody

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Abreviews
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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample Mouse Tissue sections (mouse heart)
Specification mouse heart
Permeabilization Yes - 1% triton x-100
Fixative Paraformaldehyde

Other product details

Incubation time 2 hour(s) and 0 minute(s) · Diluent: 5% goat serum
Dilution 1/200

Secondary antibody

Name Non-Abcam antibody was used: anti-mouse IgG-alexa488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution 1/400
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Submitted Jul 02 2013