Western blot abreview for Anti-COX IV [mAbcam33985] antibody

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Abreviews
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Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% SDS)
Sample Mouse Tissue lysate - whole (heart)
Specification heart
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time 24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% Tween PBS
Dilution 1/2000

Secondary antibody

Name Non-Abcam antibody was used: anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/5000

Detection

Detection method ECL
Bands Specific: 15 kDa
Exposure 1 minute(s) and 0 second(s)

Additional data

PubMed ID 23603904: View
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Submitted Aug 16 2013