Western blot abreview for Anti-COX IV [mAbcam33985] antibody

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% SDS)
Sample
Mouse Tissue lysate - whole (heart)
Specification
heart
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% Tween PBS
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 15 kDa
Exposure
1 minute(s) and 0 second(s)

Additional data

PubMed ID
23603904: View
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Submitted Aug 16 2013

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