Western blot abreview for Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker

Excellent
Abreviews
Application
Western blot
Sample
Cow Tissue lysate - other (Liver membrane)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
Liver membrane
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1.5% · Temperature: 25°C

Other product details

Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: over night
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat Anti-mouse IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 19 kDa
Positive control
Lane 1: 15ug cow liver membrane Lane 2 : 30ug cow liver membrane Lane 3 : 60ug cow liver membrane

Additional data

Additional Notes
Mixture sample is 1:1 of Basolateral and Canalicular membrane. Lane 1: 15ug cow liver membrane Lane 2 : 30ug cow liver membrane Lane 3 : 60ug cow liver membrane
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Submitted Jan 05 2018

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