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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
Product partner
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab695? Please let us know so that we can cite the reference in this datasheet
ab695 has been referenced in 1 publications.
- Ballif BA et al. Interaction of cyclooxygenases with an apoptosis- and autoimmunity-associated protein. Proc Natl Acad Sci U S A 93:5544-9 (1996). PubMed: 8643612
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](/ps/datasheet/Images/0/ab695/ab695_1.gif)
Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
Lane 1: MM standard
Lane 2: Cox-1 (protein standard prepared from ram seminal vesicles, supplied by Alpha Diagnostic International)
Antibody 5F6/F4: 1
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](/ps/datasheet/Images/0/ab695/ab695_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
ab695 at 1/400 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
![Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](/ps/datasheet/images/0/ab695/COX1-Cyclooxygenase-1-Primary-antibodies-ab695-3.jpg)
Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695) at 1 µg/ml + whole cell lysate prepared from human huh-7 cell line at 15 µg
Secondary
Sheep anti-mouse IgG conjugated to HRP at 1/1000 dilution
Performed under reducing conditions.
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Primary antibody incubated for 1 hour at 20°C in 5% milk in TBST.Gel running conditions: Denaturing.Blocked using 5% milk for 16 hours at 4°C.Detection method: Western lightning chemiluminescent reagent.
This image is courtesy of an anonymous abreview.
![COX1 / Cyclooxygenase 1 antibody [5F6/F4] for WB in Human (695)](/ps/Reviews/Images/ab8936_43705.jpg)
COX1 / Cyclooxygenase 1 antibody [5F6/F4] for WB in Human (695)
![COX1 / Cyclooxygenase 1 antibody [5F6/F4] for Western blot in Mouse (695)](/ps/datasheet/images/0/ab695/COX1-Cyclooxygenase-1-Primary-antibodies-ab695-7.jpg)
COX1 / Cyclooxygenase 1 antibody [5F6/F4] for Western blot in Mouse (695)
![COX1 / Cyclooxygenase 1 antibody [5F6/F4] for IP in Human (695)](/ps/Reviews/Images/ab9083_46083.jpg)
COX1 / Cyclooxygenase 1 antibody [5F6/F4] for IP in Human (695)
![COX1 / Cyclooxygenase 1 antibody [5F6/F4] for IHC-P in Human (695)](/ps/Reviews/Images/ab8982_43176.jpg)
COX1 / Cyclooxygenase 1 antibody [5F6/F4] for IHC-P in Human (695)
![COX1 / Cyclooxygenase 1 antibody [5F6/F4] for Immunoprecipitation in Human (695)](/ps/datasheet/images/0/ab695/COX1-Cyclooxygenase-1-Primary-antibodies-ab695-4.jpg)
COX1 / Cyclooxygenase 1 antibody [5F6/F4] for Immunoprecipitation in Human (695)
![COX1 / Cyclooxygenase 1 antibody [5F6/F4] for WB in Human (695)](/ps/datasheet/images/0/ab695/COX1-Cyclooxygenase-1-Primary-antibodies-ab695-2.jpg)
COX1 / Cyclooxygenase 1 antibody [5F6/F4] for WB in Human (695)
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