Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Is this antibody stable at low pH (2.8)? |
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ANSWER: |
Thank you for your enquiry. I am happy to forward on the reply from the originator of the antibody, which I hope you will find helpful. "Normally IgG antibodies are purified using elution buffers at lower pH of 2-3. During this purification the antibodies are stable and reactive after changing the solution to ph ~ 7. It should be possible to use ab695 under the described conditions." I hope this information helps. Please do not hesitate to contact us if you need anything further. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lane 1: MM standard
Lane 2: Cox-1 (protein standard prepared from ram seminal vesicles, supplied by Alpha Diagnostic International)
Antibody 5F6/F4: 1
ab695 at 1/400 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695) at 1 µg/ml + whole cell lysate prepared from human huh-7 cell line at 15 µg
Secondary
Sheep anti-mouse IgG conjugated to HRP at 1/1000 dilution
Performed under reducing conditions.
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Primary antibody incubated for 1 hour at 20°C in 5% milk in TBST.Gel running conditions: Denaturing.Blocked using 5% milk for 16 hours at 4°C.Detection method: Western lightning chemiluminescent reagent.
This image is courtesy of an anonymous abreview.
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