Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

Overview

• Product nameAnti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]See all COX1 / Cyclooxygenase 1 primary antibodies ...
• Description
  Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
• SpecificityCOX-1. No cross-reactivity with Cox-2.
• Tested applicationsELISA, WB, IHC-Fr, IHC-P, Flow Cyt, IP, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A preparation of intracellular proteins of the human cell line HL60.

• General notesPlease note that we have received reports from customers working with this antibody that they obtain two stong bands in Western blot. A single band at around 72kDa is expected. One customer reported that when tested with three different human cell lines two strong bands are obtained, one between 40 - 50kDa and other other between 62 - 83kDa. It is possible that a degraded form of the enzyme is detected or the bands may correspond to different glycosylation states.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS (FCS free).
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Monoclonal
• Clone number5F6/F4
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Metabolism
  • Pathways and Processes
  • Endocrine metabolism
  • Hormone biosynthesis

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipoprotein metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Hormone biosynthesis

  • Signal Transduction
  • Signaling Pathway
  • Lipid Signaling
  • Prostaglandins

  • Cardiovascular
  • Atherosclerosis
  • Lipoprotein metabolism

  • Cardiovascular
  • Blood
  • Platelets

  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

Applications

Target

• FunctionMay play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
• PathwayLipid metabolism; prostaglandin biosynthesis.
• Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
  Contains 1 EGF-like domain.
• Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.

Target information above from: UniProt accession P23219 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5742 Human
  • Entrez Gene: 5742 Human
  • Entrez Gene: 5742 Human
  • Entrez Gene: 19224 Mouse
  • Entrez Gene: 24693 Rat
  • Omim: 176805 Human
  • SwissProt: P23219 Human
  • SwissProt: P23219 Human

  • SwissProt: P23219 Human
  • SwissProt: P22437 Mouse
  • SwissProt: Q63921 Rat
  • Unigene: 201978 Human
  • Unigene: 275434 Mouse
  • Unigene: 44404 Rat

  see all

• Alternative names
  COX 1 antibodyCOX 3 antibodyCOX-1 antibody

  COX1 antibodyCOX3 antibodyCyclooxygenase 1 antibodyCyclooxygenase 3, included antibodyCyclooxygenase-1 antibodyCyclooxygenase1 antibodyEC 1.14.99.1 antibodyPartial COX1 proteins, included antibodyPCOX 1 antibodyPCOX1 antibodyPGG/HS antibodyPGH synthase 1 antibodyPGH1_HUMAN antibodyPGHS 1 antibodyPGHS-1 antibodyPGHS1 antibodyPHS 1 antibodyPHS1 antibodyProstaglandin endoperoxide synthase 1 antibodyProstaglandin G/H synthase 1 antibodyProstaglandin G/H synthase and cyclooxygenase antibodyProstaglandin H Synthase 1 antibodyProstaglandin H2 synthase 1 antibodyProstaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibodyProstaglandin-endoperoxide synthase 1 antibodyPTGHS antibodyPTGS 1 antibodyPTGS1 antibody

  see all

Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] images

• 

  Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

  Lane 1: MM standard
  Lane 2: Cox-1 (protein standard prepared from ram seminal vesicles, supplied by Alpha Diagnostic International)
  Antibody 5F6/F4: 1 µg/ml

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)This image is courtesy of an anonymous Abreview

  ab695 at 1/400 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

  See Abreview

• 

  Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
  Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695) at 1 µg/ml + whole cell lysate prepared from human huh-7 cell line at 15 µg
  
  Secondary
  Sheep anti-mouse IgG conjugated to HRP at 1/1000 dilution
  
  Performed under reducing conditions.
  
  Observed band size : 68 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 4 minutes

  This image is courtesy of an anonymous abreview.

  See Abreview

• 

  Flow Cytometry-Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4](ab695)
  Overlay histogram showing NIH3T3 cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)This image is courtesy of an anonymous Abreview
  ab695 staining COX1 / Cyclooxygenase 1 in the Mouse embryonic fibroblasts (MEF)cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% in PBS and blocked with 5% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/100 in 5% Goat serum) for 2 hours. An Alexa Fluor^®546-conjugated Goat anti-mouse IgG polyclonal(1/400) was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)This image is courtesy of an anonymous Abreview
  ab695 staining COX1 / Cyclooxygenase 1 in Mouse heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 5% Goat serum) for 2 hours. An Alexa Fluor^®488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

  See Abreview