Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

  Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

Lane 1: MM standard
Lane 2: Cox-1 (protein standard prepared from ram seminal vesicles, supplied by Alpha Diagnostic International)
Antibody 5F6/F4: 1 µg/ml

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

ab695 at 1/400 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

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  Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695) at 1 µg/ml + whole cell lysate prepared from human huh-7 cell line at 15 µg

Secondary
Sheep anti-mouse IgG conjugated to HRP at 1/1000 dilution

Performed under reducing conditions.

Observed band size : 68 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 4 minutes

Primary antibody incubated for 1 hour at 20°C in 5% milk in TBST.
Gel running conditions: Denaturing.
Blocked using 5% milk for 16 hours at 4°C.
Detection method: Western lightning chemiluminescent reagent.

This image is courtesy of an anonymous abreview.

See Abreview