Loading...
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]
See all COX1 / Cyclooxygenase 1 products (15) ...
Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
COX-1. No cross-reactivity with Cox-2.
ELISA, WB, IHC-P, IPmore details
Reacts with
Mouse, Human
A preparation of intracellular proteins of the human cell line HL60.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: PBS (FCS free).
Concentration information loading...
Immunogen affinity purified
Monoclonal
5F6/F4
IgG1
kappa
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Prostaglandins
Cardiovascular >> Blood >> Platelets
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Our Abpromise guarantee covers the use of ab695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use a concentration of 0.001 - 0.01 µg/ml.
WB: Use a concentration of 1 - 2 µg/ml.
IHC-P: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
Lipid metabolism; prostaglandin biosynthesis.
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain.
Microsome membrane. Endoplasmic reticulum membrane.
Target information above from: UniProt accessionP23219
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
![Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](/ps/datasheet/Images/0/ab695/ab695_1.gif)
Lane 1: MM standard
Lane 2: Cox-1 (protein standard prepared from ram seminal vesicles, supplied by Alpha Diagnostic International)
Antibody 5F6/F4: 1
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](/ps/datasheet/Images/0/ab695/ab695_2.jpg)
ab695 at 1/400 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
![Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](/ps/datasheet/images/0/ab695/COX1-Cyclooxygenase-1-Primary-antibodies-ab695-3.jpg)
Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695) at 1 µg/ml + whole cell lysate prepared from human huh-7 cell line at 15 µg
Secondary
Sheep anti-mouse IgG conjugated to HRP at 1/1000 dilution
Performed under reducing conditions.
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Primary antibody incubated for 1 hour at 20°C in 5% milk in TBST.Gel running conditions: Denaturing.Blocked using 5% milk for 16 hours at 4°C.Detection method: Western lightning chemiluminescent reagent.
This image is courtesy of an anonymous abreview.
This product has been referenced in:
See 1 publication for this product
Publishing research using ab695? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
![Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](/ps/datasheet/Images/0/ab695/ab695_1.gif)
Lane 1: MM standard
Lane 2: Cox-1 (protein standard prepared from ram seminal vesicles, supplied by Alpha Diagnostic International)
Antibody 5F6/F4: 1
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](/ps/datasheet/Images/0/ab695/ab695_2.jpg)
ab695 at 1/400 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
![Western blot - COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](/ps/datasheet/images/0/ab695/COX1-Cyclooxygenase-1-Primary-antibodies-ab695-3.jpg)
Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695) at 1 µg/ml + whole cell lysate prepared from human huh-7 cell line at 15 µg
Secondary
Sheep anti-mouse IgG conjugated to HRP at 1/1000 dilution
Performed under reducing conditions.
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Primary antibody incubated for 1 hour at 20°C in 5% milk in TBST.Gel running conditions: Denaturing.Blocked using 5% milk for 16 hours at 4°C.Detection method: Western lightning chemiluminescent reagent.
This image is courtesy of an anonymous abreview.
6
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Call 01223 696 000 or contact us
