(3811-1)
Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
Overview
- Product nameAnti-COX1 / Cyclooxygenase 1 antibody [EPR5866]See all COX1 / Cyclooxygenase 1 primary antibodies ...
- DescriptionRabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1
- Tested applicationsWB, IP, IHC-P, ICC/IF, Flow Cyt more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide corresponding to residues in Human COX1 / Cyclooxygenase 1.
- Positive controlNIH3T3, HACAT, Neuro -2a, C2C12, A431, and L6 cell lysates; Human skin tissue; HeLa cells.
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Dissociation constant (Kd) Kd = 5.50 x 10-12 M
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR5866
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab109025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000 - 1/10000. Predicted molecular weight: 69 kDa. |
| IP | IP: 1/10 - 1/100. |
| IHC-P | IHC-P: 1/250 - 1/500. Heat up to 98 °C, below boiling, and then let cool for 10-20 min. |
| ICC/IF | ICC/IF: 1/100 - 1/250. |
| Flow Cyt | Flow Cyt: 1/10 - 1/100. |
Target
- FunctionMay play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
- PathwayLipid metabolism; prostaglandin biosynthesis.
- Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. - Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
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Database links
- Entrez Gene: 5742 Human
- Entrez Gene: 5742 Human
- Entrez Gene: 5742 Human
- Entrez Gene: 19224 Mouse
- Entrez Gene: 24693 Rat
- Omim: 176805 Human
- SwissProt: P23219 Human
- SwissProt: P23219 Human
- SwissProt: P23219 Human
- SwissProt: P22437 Mouse
- SwissProt: Q63921 Rat
- Unigene: 201978 Human
- Unigene: 275434 Mouse
- Unigene: 44404 Rat
see all
Target information above from: UniProt accession
P23219
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- COX 1 antibodyCOX 3 antibodyCOX-1 antibody
- COX1 antibodyCox3 antibodyCyclooxygenase 1 antibodyCyclooxygenase 3, included antibodyCyclooxygenase-1 antibodyEC 1.14.99.1 antibodyPartial COX1 proteins, included antibodyPCOX1 antibodyPGG/HS antibodyPGH synthase 1 antibodyPGH1_HUMAN antibodyPGHS-1 antibodyPGHS1 antibodyPHS 1 antibodyPHS1 antibodyProstaglandin G/H synthase 1 antibodyProstaglandin H2 synthase 1 antibodyProstaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibodyProstaglandin-endoperoxide synthase 1 antibodyPTGHS antibodyPTGS1 antibody
see all
Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] images
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![Western blot - COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)](http://a.abcam.com/ps/datasheet/images/109/ab109025/COX1-Cyclooxygenase-1-Primary-antibodies-ab109025-4.JPG)
Western blot - COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/1000 dilution
Lane 1 : NIH3T3 cell lysate
Lane 2 : HACAT cell lysate
Lane 3 : Neuro 2a cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : A431 cell lysate
Lane 6 : L6 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 69 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)](http://a.abcam.com/ps/datasheet/images/109/ab109025/COX1-Cyclooxygenase-1-Primary-antibodies-ab109025-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)ab109025 at 1/250 dilution staining COX1 / Cyclooxygenase 1 in Human skin by Immunohistochemistry, Paraffin-embedded tissue. -
![Immunocytochemistry/ Immunofluorescence - COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)](http://a.abcam.com/ps/datasheet/images/109/ab109025/COX1-Cyclooxygenase-1-Primary-antibodies-ab109025-3.jpg)
Immunocytochemistry/ Immunofluorescence - COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)ab109025 at 1/100 dilution staining COX1 / Cyclooxygenase 1 in HeLa cells by Immunofluorescence. -
](http://a.abcam.com/ps/datasheet/images/109/ab109025/COX1-Cyclooxygenase-1-Primary-antibodies-ab109025-9.jpg)
Flow Cytometry-Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866](ab109025)Overlay histogram showing NIH3T3 cells stained with ab109025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109025, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
](http://a.abcam.com/ps/datasheet/images/109/ab109025/COX1-Cyclooxygenase-1-Primary-antibodies-ab109025-10.jpg)
Other-Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866](ab109025)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
References for Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
This product has been referenced in:
- McLarty JL et al. Estrogenic modulation of inflammation-related genes in male rats following volume overload. Physiol Genomics 44:362-73 (2012). WB ; Rat . Read more (PubMed: 22274565) »
