![Western blot - COX2 / Cyclooxygenase 2 antibody [5E10/D10] (ab701) image](#ab701_1.gif)
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Products:Cardiovascular >> Blood >> Platelets
Overview
Product name
Anti-COX2 / Cyclooxygenase 2 antibody [5E10/D10]
See all COX2 / Cyclooxygenase 2 products (18) ...
Description
Mouse monoclonal [5E10/D10] to COX2 / Cyclooxygenase 2
Specificity
COX-2. No cross-reactivity with Cox-1.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A preparation of intracellular proteins of the human cell line HL60.
General notes
Please note that we have received a report from a customer working with this antibody that they obtain two stong bands in WB (one between 40 - 50kDa and other other between 62 - 83kDa).
A single band at around 72kDa is expected.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS; (FCS free).
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
5E10/D10
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Prostaglandins
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Cardiovascular >> Blood >> Platelets
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Western blot - COX2 / Cyclooxygenase 2 antibody [5E10/D10] (ab701)
![Western blot - COX2 / Cyclooxygenase 2 antibody [5E10/D10] (ab701) image](/ps/datasheet/Images/0/ab701/ab701_1.gif)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.
ELISA: Use a concentration of 0.1 - 0.5 µg/ml.
Target
Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
Pathway
Lipid metabolism; prostaglandin biosynthesis.
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain.
Post-translational
modifications
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane.
Target information above from: UniProt accessionP35354
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
Database links
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Omim: 600262 Human
- SwissProt: P35354 Human
- SwissProt: Q05769 Mouse
- Unigene: 196384 Human
- Unigene: 292547 Mouse
Anti-COX2 / Cyclooxygenase 2 antibody [5E10/D10] images:
Western blot - COX2 / Cyclooxygenase 2 antibody [5E10/D10] (ab701)
Lane 1: Cox-2 (protein standard prepared from ovine placenta, supplied by Alpha Diagnostic International).
Lane 2: MM standard
Antibody 5E10/D10: 1
References for Anti-COX2 / Cyclooxygenase 2 antibody [5E10/D10] (ab701)
ab701 has not yet been referenced specifically in any publications.
Publishing research using ab701? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"