Overview
- Product nameAnti-COX2 / Cyclooxygenase 2 antibody [5E10/D10]See all COX2 / Cyclooxygenase 2 primary antibodies ...
- DescriptionMouse monoclonal [5E10/D10] to COX2 / Cyclooxygenase 2
- SpecificityCOX-2. No cross-reactivity with Cox-1.
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
A preparation of intracellular proteins of the human cell line HL60.
- General notesPlease note that we have received a report from a customer working with this antibody that they obtain two stong bands in WB (one between 40 - 50kDa and other other between 62 - 83kDa). A single band at around 72kDa is expected.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS; (FCS free). -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Monoclonal
- Clone number5E10/D10
- IsotypeIgG1
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 0.5 - 1 µg/ml. |
| ELISA | ELISA: Use a concentration of 0.1 - 0.5 µg/ml. |
Target
- FunctionMediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
- PathwayLipid metabolism; prostaglandin biosynthesis.
- Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. - Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. - Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
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Database links
- Entrez Gene: 5743 Human
- Entrez Gene: 5743 Human
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Omim: 600262 Human
- SwissProt: P35354 Human
- SwissProt: P35354 Human
- SwissProt: P35354 Human
- SwissProt: Q05769 Mouse
- Unigene: 196384 Human
- Unigene: 292547 Mouse
see all
Target information above from: UniProt accession
P35354
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- COX 2 antibodyCOX-2 antibodyCOX2 antibody
- Cyclooxygenase 2 antibodyCyclooxygenase 2b antibodyCyclooxygenase antibodyCyclooxygenase-2 antibodyCyclooxygenase2 antibodyEC 1.14.99.1 antibodyfj02a10 antibodyGlucocorticoid-regulated inflammatory cyclooxygenase antibodyGlucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibodyGRIPGHS antibodyhCox 2 antibodyMacrophage activation-associated marker protein P71/73 antibodyOTTHUMP00000033524 antibodyPES-2 antibodyPGG/HS antibodyPGH synthase 2 antibodyPGH2_HUMAN antibodyPGHS 2 antibodyPGHS-2 antibodyPGHS2 antibodyPHS 2 antibodyPHS II antibodyPHS2 antibodyProstaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibodyProstaglandin endoperoxide synthase 2 antibodyProstaglandin G/H synthase 2 antibodyProstaglandin G/H synthase 2 precursor antibodyProstaglandin G/H synthase and cyclooxygenase antibodyProstaglandin G/H synthase antibodyProstaglandin H2 synthase 2 antibodyprostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibodyProstaglandin-endoperoxide synthase 2 antibodyPTGS2 antibodyptgs2a antibodyTIS10 antibodyTIS10 protein antibodyunp1239 antibodywu:fj02a10 antibody
see all
Anti-COX2 / Cyclooxygenase 2 antibody [5E10/D10] images
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![Western blot - COX2 / Cyclooxygenase 2 antibody [5E10/D10] (ab701)](http://a.abcam.com/ps/datasheet/Images/0/ab701/ab701_1.gif)
Western blot - COX2 / Cyclooxygenase 2 antibody [5E10/D10] (ab701)Lane 1: Cox-2 (protein standard prepared from ovine placenta, supplied by Alpha Diagnostic International).
Lane 2: MM standard
Antibody 5E10/D10: 1µg/ml
References for Anti-COX2 / Cyclooxygenase 2 antibody [5E10/D10] (ab701)
ab701 has not yet been referenced specifically in any publications.
