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Products:Cardiovascular >> Blood >> Platelets
Overview
Product name
Anti-COX2 / Cyclooxygenase 2 antibody
See all COX2 / Cyclooxygenase 2 products (18) ...
Description
Rabbit polyclonal to COX2 / Cyclooxygenase 2
Tested applications
IHC-Fr, ICC/IF, Sandwich ELISA, IP, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)(Rat)
Positive control
IHC: Lung carcinoma and colon carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Prostaglandins
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Cardiovascular >> Blood >> Platelets
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Sandwich ELISA - COX2 / Cyclooxygenase 2 antibody (ab15191)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX2 / Cyclooxygenase 2 antibody (ab15191)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX2 / Cyclooxygenase 2 antibody (ab15191)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/100
ICC/IF: Use at an assay dependent dilution. (PubMed: 20660112)
sELISA: Use a concentration of 0.5 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345). (For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab90345 as Capture.)
IP: Use at an assay dependent dilution.
IHC-P: 1/200 - 1/1000.
WB: Use at an assay dependent dilution. Detects a band of approximately 69 kDa.
Target
Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
Pathway
Lipid metabolism; prostaglandin biosynthesis.
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain.
Post-translational
modifications
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane.
Target information above from: UniProt accessionP35354
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
Database links
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Entrez Gene: 29527 Rat
- Omim: 600262 Human
- SwissProt: P35354 Human
- SwissProt: Q05769 Mouse
- SwissProt: P35355 Rat
- Unigene: 196384 Human
see all
Anti-COX2 / Cyclooxygenase 2 antibody images:
Sandwich ELISA - COX2 / Cyclooxygenase 2 antibody (ab15191)
Standard Curve for COX2 / Cyclooxygenase 2 (Analyte: ab58868) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345) at 1ug/ml and Detector Antibody Rabbit polyclonal to COX2 / Cyclooxygenase 2 (ab15191) at 0.5ug/ml
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX2 / Cyclooxygenase 2 antibody (ab15191)
ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX2 / Cyclooxygenase 2 antibody (ab15191)
ab15191 staining mouse brain sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT. The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotin
This image is courtesy of an Abreview submitted by Mr Carl Hobbs
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX2 / Cyclooxygenase 2 antibody (ab15191)
ab15191 staining adult rat hippocampus tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval (in citric acid, pH6, 10mM) prior to blocking in 1% BSA for 10 minutes at RT. The primary anitbody was diluted 1/400 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit antibody, diluted 1/300 was used as the secondary.
Clear cytoplasmic staining in a subset of neurones in the dentate gyrus of a sagittal section of whole rat brain can be seen. In other areas of this section, there are, additionally, small cells and their processes that are positive: they may be inter-neurones or microglia: it would be neccessary to carry out double-immunostaining to confirm this.
This image is courtesy of an Abreview submitted by Mr Carl Hobbs
Immunohistochemistry (Frozen sections) - COX2 / Cyclooxygenase 2 antibody (ab15191)
ab15191 staining tissue sections of arteriosclerotic plaque in mouse aorta by IHC-Fr. Sections were acetone fixed and blocked in 1% serum for 10 minutes at 20°C prior to incubation with the primary antibody (diluted 1/500) for 1 hour at 20°C. A biotinylated donkey anti-rabbit antibody (diluted 1/500) was used as the secondary.
This image is courtesy of an Abreview submitted by Miss Silke Vorwald
Protocols
References for Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
This product has been referenced in:
- Kim Jet al. Mitochondrial loss, dysfunction and altered dynamics in Huntington's disease. Hum Mol Genet 19:3919-35 (2010). ICC/IF; Human.Read more (PubMed: 20660112) »
- Broom OJet al. CD47 regulates collagen I-induced cyclooxygenase-2 expression and intestinal epithelial cell migration. PLoS One 4:e6371 (2009).Read more (PubMed: 19636412) »
See all 7 publications for this product
Publishing research using ab15191? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"