Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
Key features and details
- Rabbit polyclonal to COX2 / Cyclooxygenase 2
- Suitable for: WB, Sandwich ELISA, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-COX2 / Cyclooxygenase 2 antibody
See all COX2 / Cyclooxygenase 2 primary antibodies -
Description
Rabbit polyclonal to COX2 / Cyclooxygenase 2 -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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Tested applications
Suitable for: WB, Sandwich ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Syrian hamster -
Immunogen
Synthetic peptide within Rat COX2/ Cyclooxygenase 2 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P35355 -
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (19) |
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
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Sandwich ELISA |
Use a concentration of 0.5 µg/ml.
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab90345 as Capture. |
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IHC-P | (8) |
1/100.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa. |
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab90345 as Capture. |
IHC-P
1/100. |
Target
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Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Entrez Gene: 29527 Rat
- Omim: 600262 Human
- SwissProt: P35354 Human
- SwissProt: Q05769 Mouse
- SwissProt: P35355 Rat
- Unigene: 196384 Human
see all -
Alternative names
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
Images
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All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab15191) at 1 µg/ml
Lane 1 : Recombinant human COX2 / Cyclooxygenase 2 protein (Active) (ab58868) at 0.1 µg
Lane 2 : Recombinant human COX2 / Cyclooxygenase 2 protein (Active) (ab58868) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Exposure time: 10 seconds -
ab15191 staining mouse brain sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT. The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotin
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ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (394)
ab15191 has been referenced in 394 publications.
- Lee H et al. Protective Effect of Gamma Aminobutyric Acid against Aggravation of Renal Injury Caused by High Salt Intake in Cisplatin-Induced Nephrotoxicity. Int J Mol Sci 23:N/A (2022). PubMed: 35008928
- Liu TT et al. Efficacy profile of noninvasive vagus nerve stimulation on cortical spreading depression susceptibility and the tissue response in a rat model. J Headache Pain 23:12 (2022). PubMed: 35062860
- Qiao W et al. Divalent metal cations stimulate skeleton interoception for new bone formation in mouse injury models. Nat Commun 13:535 (2022). PubMed: 35087048
- De Matteis R et al. Aspirin activates resolution pathways to reprogram T cell and macrophage responses in colitis-associated colorectal cancer. Sci Adv 8:eabl5420 (2022). PubMed: 35108049
- Du X et al. Circular RNA hsa_circ_0083756 promotes intervertebral disc degeneration by sponging miR-558 and regulating TREM1 expression. Cell Prolif 55:e13205 (2022). PubMed: 35187741