COX2 / Cyclooxygenase 2 antibody (ab6665)

Product Name 

COX2 / Cyclooxygenase 2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to COX2 / Cyclooxygenase 2

Immunogen 

Recombinant fusion protein (Human C-terminal end)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

The antibody does not cross react with Cox-1.

Epitope 

carboxy-terminus of human Cox-2.

Tested applications 
(see key)

Dot, ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression.
This antiserum against Human Cox-2 and has been tested for use in immunoblotting (1:5,000 to 1:10,000) and ELISA (1:10,000 to 1:50,000).
The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b.

Cellular localization 

Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Prostaglandins
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Cardiovascular >> Blood >> Platelets

Relevance 

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

85.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

COX-2, also known as prostaglandin H synthase, was identified less than a decade ago. Its discovery was followed by a period of discovery and drug development to create a “super aspirin” and led to insights into arthritis, Alzheimer's disease, colorectal cancer and regulation of brain and kidney function in search of better treatments for degenerative and inflammatory diseases. Two different isoforms of the enzyme are known, Cox-1 and Cox-2.

References for COX2 / Cyclooxygenase 2 antibody (ab6665)

  This product has been used in: (two most recent references)

Jim nez-Jim nez R et al. Eccentric training impairs NF-kappaB activation and over-expression of inflammation-related genes induced by acute eccentric exercise in the elderly. Mech Ageing Dev 129:313-21 (2008). PubMed: 18377953

Odau S et al. Differential expression of cyclooxygenase 1 and cyclooxygenase 2 in the bovine oviduct. J Endocrinol 191:263-74 (2006). PubMed: 17065409

See all 5 publication references for this product.


If you publish research using ab6665 please let us know so that we can cite the reference on this datasheet.

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