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Products:Cardiovascular >> Blood >> Platelets

Anti-COX2 / Cyclooxygenase 2 antibody (ab6665)

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Overview

Product name

Anti-COX2 / Cyclooxygenase 2 antibody
See all COX2 / Cyclooxygenase 2 products (18) ...

Description

Rabbit polyclonal to COX2 / Cyclooxygenase 2

Specificity

The antibody does not cross react with Cox-1.

Tested applications

WB, ELISA, Dot Blotmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Recombinant fusion protein (Human C-terminal end)

Epitope

carboxy-terminus of human Cox-2.

General notes

COX-2, also known as prostaglandin H synthase, was identified less than a decade ago. Its discovery was followed by a period of discovery and drug development to create a “super aspirin” and led to insights into arthritis, Alzheimer's disease, colorectal cancer and regulation of brain and kidney function in search of better treatments for degenerative and inflammatory diseases. Two different isoforms of the enzyme are known, Cox-1 and Cox-2.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Prostaglandins
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Cardiovascular >> Blood >> Platelets

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

  • ELISA

     

  • Dot Blot

     

Application notes

Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression.
This antiserum against Human Cox-2 and has been tested for use in immunoblotting (1:5,000 to 1:10,000) and ELISA (1:10,000 to 1:50,000).
The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b.

Target

Function

Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.

Pathway

Lipid metabolism; prostaglandin biosynthesis.

Sequence similarities

Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain.

Post-translational
modifications

S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.

Cellular localization

Microsome membrane. Endoplasmic reticulum membrane.

Target information above from: UniProt accessionP35354 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • COX 2 antibody
  • COX-2 antibody
  • COX2 antibody
  • Cyclooxygenase 2 antibody
  • Cyclooxygenase 2b antibody
  • Cyclooxygenase antibody
  • Cyclooxygenase-2 antibody
  • Cyclooxygenase2 antibody
  • EC 1.14.99.1 antibody
  • fj02a10 antibody
  • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
  • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
  • GRIPGHS antibody
  • hCox 2 antibody
  • Macrophage activation-associated marker protein P71/73 antibody
  • OTTHUMP00000033524 antibody
  • PES-2 antibody
  • PGG/HS antibody
  • PGH synthase 2 antibody
  • PGH2_HUMAN antibody
  • PGHS 2 antibody
  • PGHS-2 antibody
  • PGHS2 antibody
  • PHS 2 antibody
  • PHS II antibody
  • PHS2 antibody
  • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
  • Prostaglandin endoperoxide synthase 2 antibody
  • Prostaglandin G/H synthase 2 antibody
  • Prostaglandin G/H synthase 2 precursor antibody
  • Prostaglandin G/H synthase and cyclooxygenase antibody
  • Prostaglandin G/H synthase antibody
  • Prostaglandin H2 synthase 2 antibody
  • Prostaglandin-endoperoxide synthase 2 antibody
  • PTGS2 antibody
  • ptgs2a antibody
  • TIS10 antibody
  • TIS10 protein antibody
  • unp1239 antibody
  • wu:fj02a10 antibody
see all

Database links

see all

References for Anti-COX2 / Cyclooxygenase 2 antibody (ab6665)

This product has been referenced in:

  • Cuevas MJet al. Suppression of amphiregulin/epidermal growth factor receptor signals contributes to the protective effects of quercetin in cirrhotic rats. J Nutr 141:1299-305 (2011).Read more (PubMed: 21562239) »
  • García-Lastra Ret al. Signaling pathways involved in liver injury and regeneration in rabbit hemorrhagic disease, an animal model of virally-induced fulminant hepatic failure. Vet Res 41:2 (2010). WB; Mouse.Read more (PubMed: 19726019) »

See all 6 publications for this product

Publishing research using ab6665? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"