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Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)

MSCatalog No. MS408

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Overview

Product name

Anti-COX4 + COX4L2 antibody [10G8D12C12 ]
See all COX4 + COX4L2 products (2) ...

Description

Mouse monoclonal [10G8D12C12 ] to COX4 + COX4L2

Tested applications

IHC-Fr, WB, Flow Cyt, ICC/IF, IHC-Pmore details

Cross reactivity

Reacts with

Cow, Human

Immunogen

Full length native Bovine protein, COX4 + COX4L2.

Positive control

Isolated mitochondria from Human heart, Bovine heart and HepG2 cells. Human skeletal muscle (frozen sections). Cultured Human embryonic lung derived fibroblasts (strain MRC5; fixed and permeabilized). Human colon tissue sections. HeLa cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HBS

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

ab110261 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110261 was judged as near homogeneity by SDS PAGE.

Clonality

Monoclonal

Clone number

10G8D12C12

Isotype

IgG2a

Light chain type

kappa

Research areas

Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex IV
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers

  • Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261) image (enlarge)

  • Flow Cytometry - COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)Flow Cytometry - COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab110261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Cytochrome C Oxidase is located in the inner mitochondrial membrane and is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced cytochrome C and donates them to molecular oxygen, which is then reduced to water. It is composed of cytochrome A and cytochrome B, two copper atoms, and 13 different protein subunits, three of which are encoded by the mitochondrial DNA and ten others by nuclear DNA (mammals).

Cellular localization

Mitochondrial inner membrane

Alternative names

  • Cox4 antibody
  • Cox4a antibody
  • AL024441 antibody
  • COX 4 antibody
  • COX IV antibody
  • COX4B antibody
  • COX4I1 antibody
  • COX4I2 antibody
  • COX4L2 antibody
  • COXIV antibody
  • Cytochrome c oxidase polypeptide IV antibody
  • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
  • Cytochrome C Oxidase subunit IV antibody
  • Cytochrome c oxidase subunit IV isoform 1 antibody
  • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
  • Cytochrome c oxydase subunit 4 antibody
  • dJ857M17.2 antibody
  • MGC105470 antibody
  • MGC72016 antibody
  • MS408 antibody
see all

Database links

Anti-COX4 + COX4L2 antibody [10G8D12C12 ] images:

  Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)

Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)

All lanes : Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261) at 1 µg/ml

Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from HepG2 at 20 µg


Predicted band size : 20 kDa

  Flow Cytometry - COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)

Flow Cytometry - COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)

ab110261, at 1 µg/mL, staining COX4 + COX4L2 in HeLa cells by Immunohistochemistry (blue) and an isotype control antibody, at 1 µg/mL, staining of HeLa cells (red).

  Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)

Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)

ab110261, at 10 µg/ml, staining COX4 + COX4L2 in cultured Human embryonic lung derived fibroblasts by Immunofluorescence. The cells (strain MRC5) were fixed, permeabilized and then labeled with ab110261 followed by an AlexaFluor® 488 conjugated Goat anti-mouse IgG2a isotype specific secondary antibody at 1 µg/ml.

  Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)

Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)

ab110261, at 1/800, staining COX4 + COX4L2 in skeletal muscle tissue sections from a patient with a single large deletion of the mtDNA by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.

  Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)

Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)

ab110261, at 1/200, showing COX4 + COX4L2 negative crypts in colon tissue sections from a normal ageing patient by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.

References for Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)

This product has been referenced in:

  • Schmidt H  et al. Enrichment and analysis of secretory lysosomes from lymphocyte populations. BMC Immunol 10:41 (2009).Read more (PubMed: 19640298) »
  • Trougakos IP  et al. Intracellular clusterin inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein complex. Clin Cancer Res 15:48-59 (2009).Read more (PubMed: 19118032) »

See all 25 publications for this product

Publishing research using ab110261? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"