Overview

  • Product name
    Anti-COX5A antibody [6E9B12D5]
    See all COX5A primary antibodies
  • Description
    Mouse monoclonal [6E9B12D5] to COX5A
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Zebrafish
  • Immunogen

    Cow COX5A

  • Positive control
    • Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

     

    Alternative versions available:

    Anti-COX5A antibody (HRP) [6E9B12D5] (ab198040)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: HBS
  • Concentration information loading...
  • Purification notes
    ab110262 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • Clonality
    Monoclonal
  • Clone number
    6E9B12D5
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22748147
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

Anti-COX5A antibody [6E9B12D5] images

  • All lanes : Anti-COX5A antibody [6E9B12D5] (ab110262) at 1 µg/ml

    Lane 1 : Human heart mitochondria at 5 µg
    Lane 2 : Bovine heart mitochondria at 1 µg
    Lane 3 : Rat heart mitochondria at 10 µg
    Lane 4 : Mouse heart mitochondria at 10 µg
    Lane 5 : HepG2 mitochondria at 20 µg


    Predicted band size : 17 kDa
  • Mitochondrial localization of COX5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110262 (10 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (1 µg/ml).
  • HeLa cells were stained with 1 µg/mL ab110262 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

References for Anti-COX5A antibody [6E9B12D5] (ab110262)

This product has been referenced in:
  • Nam M  et al. Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression. Sci Rep 7:2013 (2017). Read more (PubMed: 28515438) »
  • Gómez-Serrano M  et al. Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes. Redox Biol 11:415-428 (2017). Human . Read more (PubMed: 28064117) »

See all 36 Publications for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab110261.
To check the status of the order please contact our Cu...

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Thanks for sending the western blot data to my Outlook account. I agree it is very strange that the powerpoint file somehow turned into one of our datasheets!
I agree that the vial of ab110262 you received is not working as expcted. I am happy to ...

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Thanks for your reply.
Oddly, the powerpoint attachement opens up to show an image of one of our datasheets, for ab110262. Not sure what the cause is but if you could, please forward the powerpoint image to my outlook account.

Thank you for your enquiry. I am sorry to hear this vial of ab110262 is giving you trouble.
And thanks for including the western blot images. If I understand you correctly, you first probed the membrane with anti-CORE2 antibody and then probed the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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