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MitoSciences (MS409)

Anti-COX5A antibody [6E9B12D5 ] (ab110262)

Submit an Abreview Q&A (4)Specific References (26)

Overview

  • Product nameAnti-COX5A antibody [6E9B12D5 ]See all COX5A primary antibodies ...
  • Description
    Mouse monoclonal [6E9B12D5 ] to COX5A
  • Tested applicationsIHC-P, WB, ICC/IF, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Zebrafish
  • Immunogen

    Cow COX5A

  • Positive controlIsolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C. Do not freeze.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: HBS
  • Concentration information loading...
  • Purification notesab110262 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • Clonality Monoclonal
  • Clone number6E9B12D5
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab110262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: Use at an assay dependent concentration. PubMed: 22748147
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
ICC/IF ICC/IF: Use a concentration of 5 µg/ml.
Flow Cyt Flow Cyt: Use a concentration of 1 µg/ml.

Target

  • Alternative names
      COX antibodyCOX VA antibodyCOX5A antibody
      COX5A_HUMAN antibodyCytochrome c oxidase polypeptide Va antibodyCytochrome c oxidase polypeptide, mitochondrial antibodyCytochrome c oxidase subunit 5A antibodyCytochrome c oxidase subunit 5A, mitochondrial antibodymitochondrial antibodyMitochondrial cytochrome c oxidase subunit Va antibodyVA antibody
    see all

Anti-COX5A antibody [6E9B12D5 ] images

  • Western blot - Anti-COX5A antibody [6E9B12D5 ] (ab110262)
    Western blot - Anti-COX5A antibody [6E9B12D5 ] (ab110262)
    All lanes : Anti-COX5A antibody [6E9B12D5 ] (ab110262) at 1 µg/ml

    Lane 1 : Human heart mitochondria at 5 µg
    Lane 2 : Bovine heart mitochondria at 1 µg
    Lane 3 : Rat heart mitochondria at 10 µg
    Lane 4 : Mouse heart mitochondria at 10 µg
    Lane 5 : HepG2 mitochondria at 20 µg


    Predicted band size : 17 kDa
  • Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5 ] (ab110262)
    Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5 ] (ab110262)
    Mitochondrial localization of COX5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110262 (10 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (1 µg/ml).
  • Flow Cytometry - COX5A antibody [6E9B12D5 ] (ab110262)
    Flow Cytometry - COX5A antibody [6E9B12D5 ] (ab110262)
    HeLa cells were stained with 1 µg/mL ab110262 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

References for Anti-COX5A antibody [6E9B12D5 ] (ab110262)

This product has been referenced in:
  • Chen WL  et al. The role of cytochrome c oxidase subunit Va in non-small cell lung carcinoma cells: association with migration, invasion and prediction of distant metastasis. BMC Cancer 12:273 (2012). WB, IHC-P ; Human . Read more (PubMed: 22748147) »
  • Atkinson A  et al. Mzm1 influences a labile pool of mitochondrial zinc important for respiratory function. J Biol Chem 285:19450-9 (2010). Read more (PubMed: 20404342) »

See all 26 Publications for this product

Publishing research using ab110262 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab110262 Submit a Question for ab110262

Displaying 1 - 4 of 4 results for Abreviews and Q&A

10
Votes: Highest First

Thank you, we look forward to receiving the replacement. The lot you have available, GR47109-2, will be fine.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab110261.
To check the status of the order please contact our Custo...

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Dear Technical Support/Custom Service,



We purchased ab110262 on March 26, 2012, and unfortunately it did not perform in our experiments. Please see attached image. Would it be possible to receive a substitution/replacement (MS408 CO...

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Thanks for sending the western blot data to my Outlook account. I agree it is very strange that the powerpoint file somehow turned into one of our datasheets!
I agree that the vial of ab110262 you received is not working as expcted. I am happy to sen...

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Please see the attached image of another experiment with a negative
result that we obtained with Ab110262 COX5a lot GR73589-1. Moreover, we don't
need to strip the membranes prior to the second blot (with CORE2), since
the two prot...

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Thanks for your reply.
Oddly, the powerpoint attachement opens up to show an image of one of our datasheets, for ab110262. Not sure what the cause is but if you could, please forward the powerpoint image to my outlook account.

Dear Technical Support/Custom Service,



We purchased ab110262 on March 26, 2012 (Order No. 1059903), and unfortunately it did not perform in our experiments. Please see attached image. Would it be possible to receive a substitution/r...

Read More

Thank you for your enquiry. I am sorry to hear this vial of ab110262 is giving you trouble.
And thanks for including the western blot images. If I understand you correctly, you first probed the membrane with anti-CORE2 antibody and then probed the sa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"