![Western blot - COX6A1 antibody [14A3AD2BH4 ] (ab110265) image](#COX6A1-Primary-antibodies-ab110265-1.jpg)
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Product name
Anti-COX6A1 antibody [14A3AD2BH4 ]
See all COX6A1 products (3) ...
Description
Mouse monoclonal [14A3AD2BH4 ] to COX6A1
Specificity
ab110265 recognises the Liver isoform in human, rat, mouse.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Rabbit, Cow, Dog
Immunogen
Synthetic peptide: SSGAHGEEGSARMWKALTLF(C), corresponding to amino acids 25-44 of Cow COX6A1
SSGAHGEEGSARMWKALTLF(C)
Positive control
HepG2, Human, Mouse and Rat heart lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purification notes
ab110265 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
14A3AD2BH4
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex IV
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Western blot - COX6A1 antibody [14A3AD2BH4 ] (ab110265)
![Western blot - COX6A1 antibody [14A3AD2BH4 ] (ab110265) image](/ps/datasheet/images/110/ab110265/COX6A1-Primary-antibodies-ab110265-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab110265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 5 µg/ml. Predicted molecular weight: 12 kDa.
Target
Function
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Sequence similarities
Belongs to the cytochrome c oxidase subunit 6A family.
Cellular localization
Mitochondrion inner membrane.
Target information above from: UniProt accessionP12074
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- COX VIa L antibody
- COX VIa-L antibody
see all
Database links
- Entrez Gene: 1337 Human
- Entrez Gene: 12861 Mouse
- Entrez Gene: 25282 Rat
- Omim: 602072 Human
- SwissProt: P12074 Human
- SwissProt: P43024 Mouse
- SwissProt: P10818 Rat
- Unigene: 497118 Human
see all
Anti-COX6A1 antibody [14A3AD2BH4 ] images:
Western blot - COX6A1 antibody [14A3AD2BH4 ] (ab110265)
All lanes : Anti-COX6A1 antibody [14A3AD2BH4 ] (ab110265) at 5 µg/ml
Lane 1 : Human Heart Lysate
Lane 2 : Rat Heart Lysate
Lane 3 : Mouse Heart Lysate
Lane 4 : HepG2 cell lysate
Predicted band size : 12 kDa
Extra bands in the mouse sample (lane 3) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
References for Anti-COX6A1 antibody [14A3AD2BH4 ] (ab110265)
This product has been referenced in:
- Sohal RS et al. Age-related decrease in expression of mitochondrial DNA encoded subunits of cytochrome c oxidase in Drosophila melanogaster. Mech Ageing Dev 129:558-61 (2008).Read more (PubMed: 18538373) »
See 1 publication for this product
Publishing research using ab110265? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"