Anti-COX IV antibody [4D11-B3-E8] (ab131177)

Overview

• Product nameAnti-COX IV antibody [4D11-B3-E8]See all COX IV primary antibodies ...
• Description
  Mouse monoclonal [4D11-B3-E8] to COX IV
• Specificityab131177 detects endogenous levels of COXIV Isoform 1 and does not cross-react with related proteins.
• Tested applicationsICC/IF, WB, ELISA, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide of Human COXIV Isoform 1.

• Positive controlHeLa cells; Mouse skeletal muscle and Jurkat cell lysates; K562 cells for flow cytometry.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.2% Sodium azide
  Constituents: 49% Tris glycine, 50% Glycerol, 0.88% Sodium chloride
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number4D11-B3-E8
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Oxidative phosphorylation
  • Complex IV

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Isotype/Loading Controls
  • Loading Controls
  • COX IV - Mitochondrial

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

Applications

Target

• FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the cytochrome c oxidase IV family.
• Cellular localizationMitochondrion inner membrane.

Target information above from: UniProt accession P13073 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1327 Human
  • Entrez Gene: 1327 Human
  • Omim: 123864 Human
  • SwissProt: P13073 Human
  • SwissProt: P13073 Human
  • Unigene: 433419 Human

• Alternative names
  AL024441 antibodyCOX 4 antibodyCOX IV 1 antibody

  COX IV antibodyCOX IV-1 antibodyCox4 antibodyCOX41_HUMAN antibodyCox4a antibodyCOX4B antibodyCOX4I1 antibodyCOX4I2 antibodyCOX4L2 antibodyCOXIV antibodyCytochrome c oxidase polypeptide IV antibodyCytochrome c oxidase subunit 4 isoform 1 mitochondrial antibodyCytochrome c oxidase subunit 4 isoform 1, mitochondrial antibodyCytochrome C Oxidase subunit IV antibodyCytochrome c oxidase subunit IV isoform 1 antibodyCytochrome c oxidase subunit IV isoform 2 (lung) antibodyCytochrome c oxydase subunit 4 antibodydJ857M17.2 antibodyMGC105470 antibodyMGC72016 antibody

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Anti-COX IV antibody [4D11-B3-E8] images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-COXIV Isoform 2 antibody (ab131177)
  Immunofluorescent analysis of HeLa cells labelling COXIV Isoform 2 with ab131177 at 1/150 dilution.
• 

  Western blot - Anti-COXIV Isoform 2 antibody (ab131177)
  All lanes : Anti-COX IV antibody [4D11-B3-E8] (ab131177) at 1/1000 dilution
  
  Lane 1 : Mouse skeletal muscle lysate
  Lane 2 : Jurkat cell lysate
  
  
  Predicted band size : 20 kDa
  
• 

  Flow Cytometry - Anti-COXIV Isoform 2 antibody (ab131177)
  Flow Cytometric analysis of K562 cells labelling COXIV Isoform 2 with ab131177 (red) at 1/100 dilution; followed by FITC-conjugated goat anti-mouse IgG. Mouse IgG control (blue).