The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
1/500 - 1/1000.
FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Tissue specificityHighly expressed in lung.
Involvement in diseaseDefects in COX4I2 are the cause of exocrine pancreatic insufficiency dyserythropoietic anemia and calvarial hyperostosis (EPIDACH) [MIM:612714]. Patients present with pancreatic insufficiency, intestinal malabsorption, failure to thrive, and anemia soon after birth.
Sequence similaritiesBelongs to the cytochrome c oxidase IV family.
ab70112 at 1/500 dilution staining COXIV Isoform 2 in COS7 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Western blot - COXIV Isoform 2 antibody (ab70112)
All lanes : Anti-COXIV Isoform 2 antibody (ab70112) at 1/500 dilution
Lane 1 : K562 cell extracts treated
with insulin (0.01U/ml, 15mins) Lane 2 : K562 cell extracts treated
with insulin (0.01U/ml, 15mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 20 kDa Observed band size : 20 kDa
References for Anti-COXIV Isoform 2 antibody (ab70112)
This product has been referenced in:
Lou PH et al. Loss of Intralipid®- but not sevoflurane-mediated cardioprotection in early type-2 diabetic hearts of fructose-fed rats: importance of ROS signaling. PLoS One9:e104971 (2014).
Read more (PubMed: 25127027) »
Head B et al. Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells. J Cell Biol187:959-66 (2009).
Read more (PubMed: 20038677) »