Anti-COXIV Isoform 2 antibody (ab70112)

Overview

• Product nameAnti-COXIV Isoform 2 antibodySee all COXIV Isoform 2 primary antibodies ...
• Description
  Rabbit polyclonal to COXIV Isoform 2
• Specificityab70112 detects endogenous levels of total COXIV Isoform 2 protein.
• Tested applicationsELISA, WB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat
• Immunogen

  Synthetic peptide derived from an internal sequence of human COXIV Isoform 2

• Positive controlCOS7 cells and K562 cell extracts treated with insulin (0.01U/ml, 15mins).

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab70112 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Oxidative phosphorylation
  • Complex IV

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Isotype/Loading Controls
  • Loading Controls
  • COX IV - Mitochondrial

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

Applications

Target

• FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
• Tissue specificityHighly expressed in lung.
• Involvement in diseaseDefects in COX4I2 are the cause of exocrine pancreatic insufficiency dyserythropoietic anemia and calvarial hyperostosis (EPIDACH) [MIM:612714]. Patients present with pancreatic insufficiency, intestinal malabsorption, failure to thrive, and anemia soon after birth.
• Sequence similaritiesBelongs to the cytochrome c oxidase IV family.
• Cellular localizationMitochondrion inner membrane.

Target information above from: UniProt accession Q96KJ9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 84701 Human
  • Entrez Gene: 84701 Human
  • Entrez Gene: 84701 Human
  • Entrez Gene: 84701 Human
  • Entrez Gene: 84682 Mouse
  • Entrez Gene: 84683 Rat
  • Omim: 607976 Human
  • SwissProt: Q96KJ9 Human

  • SwissProt: Q96KJ9 Human
  • SwissProt: Q96KJ9 Human
  • SwissProt: Q96KJ9 Human
  • SwissProt: Q91W29 Mouse
  • SwissProt: Q91Y94 Rat
  • Unigene: 277101 Human
  • Unigene: 196668 Mouse
  • Unigene: 7214 Rat

  see all

• Alternative names
  COX IV-2 antibodyCOX4 2 antibodyCOX4 antibody

  COX42_HUMAN antibodyCOX4B antibodyCox4i2 antibodyCOX4L2 antibodyCOXIV 2 antibodyCytochrome c oxidase subunit 4 isoform 2 antibodyCytochrome c oxidase subunit 4 isoform 2 mitochondrial [Precursor] antibodycytochrome c oxidase subunit IV isoform 2 (lung) antibodyCytochrome c oxidase subunit IV isoform 2 antibodydJ857M17.2 antibodymitochondrial antibodyRP23 106A3.3 antibody

  see all

Anti-COXIV Isoform 2 antibody images

• 

  Immunocytochemistry/ Immunofluorescence - COXIV Isoform 2 antibody (ab70112)
  ab70112 at 1/500 dilution staining COXIV Isoform 2 in COS7 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
• 

  Western blot - COXIV Isoform 2 antibody (ab70112)
  All lanes : Anti-COXIV Isoform 2 antibody (ab70112) at 1/500 dilution
  
  Lane 1 : K562 cell extracts treated with insulin (0.01U/ml, 15mins)
  Lane 2 : K562 cell extracts treated with insulin (0.01U/ml, 15mins) with immunising peptide at 10 µg
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 20 kDa
  Observed band size : 20 kDa