Overview

  • Product nameAnti-COXIV Isoform 2 antibody
    See all COXIV Isoform 2 primary antibodies
  • Description
    Rabbit polyclonal to COXIV Isoform 2
  • Specificityab70112 detects endogenous levels of total COXIV Isoform 2 protein.
  • Tested applicationsELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human COXIV Isoform 2

  • Positive control
    • COS7 cells and K562 cell extracts treated with insulin (0.01U/ml, 15mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab70112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF 1/500 - 1/1000.

Target

  • FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Tissue specificityHighly expressed in lung.
  • Involvement in diseaseDefects in COX4I2 are the cause of exocrine pancreatic insufficiency dyserythropoietic anemia and calvarial hyperostosis (EPIDACH) [MIM:612714]. Patients present with pancreatic insufficiency, intestinal malabsorption, failure to thrive, and anemia soon after birth.
  • Sequence similaritiesBelongs to the cytochrome c oxidase IV family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX IV-2 antibody
    • COX4 2 antibody
    • COX4 antibody
    • COX42_HUMAN antibody
    • COX4B antibody
    • Cox4i2 antibody
    • COX4L2 antibody
    • COXIV 2 antibody
    • Cytochrome c oxidase subunit 4 isoform 2 antibody
    • Cytochrome c oxidase subunit 4 isoform 2 mitochondrial [Precursor] antibody
    • cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxidase subunit IV isoform 2 antibody
    • dJ857M17.2 antibody
    • mitochondrial antibody
    • RP23 106A3.3 antibody
    see all

Anti-COXIV Isoform 2 antibody images

  • ab70112 at 1/500 dilution staining COXIV Isoform 2 in COS7 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-COXIV Isoform 2 antibody (ab70112) at 1/500 dilution

    Lane 1 : K562 cell extracts treated with insulin (0.01U/ml, 15mins)
    Lane 2 : K562 cell extracts treated with insulin (0.01U/ml, 15mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 20 kDa
    Observed band size : 20 kDa

References for Anti-COXIV Isoform 2 antibody (ab70112)

This product has been referenced in:
  • Head B  et al. Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells. J Cell Biol 187:959-66 (2009). Read more (PubMed: 20038677) »
  • Ahn BH  et al. A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proc Natl Acad Sci U S A 105:14447-52 (2008). WB ; Mouse . Read more (PubMed: 18794531) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab70112.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"