Anti-CPT1A antibody [8F6AE9] (ab128568)

Overview

• Product nameAnti-CPT1A antibody [8F6AE9]See all CPT1A primary antibodies ...
• Description
  Mouse monoclonal [8F6AE9] to CPT1A
• Tested applicationsIHC-P, WB, ICC/IF, In-Cell ELISA more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Recombinant fragment, corresponding to amino acids 489-773 of Human CPT1A.

• Positive controlPartial Human Recombinant CPT1A protein; HepG2, H9C2, H4IIE Whole Cell Lysates; Rat heart mitochondrial, Human liver, Rat liver and Mouse liver homogenates; H9C2 cells; HeLa cells IHC-P: human normal kidney FFPE tissue sections

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferPreservative: 0.02% Sodium azide
  Constituent: 99% HBS
• Concentration information loading...
• PurityAmmonium Sulphate Precipitation
• Purification notesPurity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
• Clonality Monoclonal
• Clone number8F6AE9
• IsotypeIgG2b
• Light chain typekappa
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Fatty acids

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Metabolism

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

Applications

Target

• Tissue specificityStrong expression in kidney and heart, and lower in liver and skeletal muscle.
• PathwayLipid metabolism; fatty acid beta-oxidation.
• Involvement in diseaseDefects in CPT1A are the cause of carnitine palmitoyltransferase 1A deficiency (CPT1AD) [MIM:255120]; also known as CPT-I deficiency or CPT1A deficiency. CPT1AD is a rare autosomal recessive metabolic disorder of long-chain fatty acid oxidation characterized by severe episodes of hypoketotic hypoglycemia usually occurring after fasting or illness. Onset is in infancy or early childhood.
• Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
• Cellular localizationMitochondrion outer membrane.

Target information above from: UniProt accession P50416 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1374 Human
  • Entrez Gene: 12894 Mouse
  • Entrez Gene: 25757 Rat
  • Omim: 600528 Human
  • SwissProt: P50416 Human
  • SwissProt: P97742 Mouse
  • SwissProt: P32198 Rat
  • Unigene: 503043 Human

  • Unigene: 18522 Mouse
  • Unigene: 2856 Rat

  see all

• Alternative names
  Carnitine O palmitoyltransferase 1 liver isoform antibodyCarnitine O palmitoyltransferase I antibodyCarnitine O palmitoyltransferase I liver isoform antibody

  Carnitine O-palmitoyltransferase 1 antibodyCarnitine O-palmitoyltransferase I antibodyCarnitine palmitoyltransferase 1A (liver) antibodyCarnitine palmitoyltransferase 1A antibodyCarnitine palmitoyltransferase I antibodyCarnitine palmitoyltransferase I liver antibodyCPT 1 antibodyCPT I antibodyCPT1 antibodyCPT1 L antibodyCPT1-L antibodyCpt1a antibodyCPT1A_HUMAN antibodyCPTI antibodyCPTI-L antibodyL CPT1 antibodyliver isoform antibody

  see all

Anti-CPT1A antibody [8F6AE9] images

• 

  Western blot - Anti-CPT1A antibody (ab128568)
  All lanes : Anti-CPT1A antibody [8F6AE9] (ab128568) at 1 µg/ml
  
  Lane 1 : Marker
  Lane 2 : Partial Human Recombinant CPT1A protein (ab128569) at 0.1 µg
  Lane 3 : Full Length Human Recombinant OTC protein at 0.1 µg
  Lane 4 : HepG2 (Human hepatocellular carcinoma cell line) Whole Cell Lysate at 20 µg
  Lane 5 : H9C2 (Rat cardiomyoblast cell line) Whole Cell Lysate at 20 µg
  Lane 6 : H4IIE ( Rat hepatoma cell line) Whole Cell Lysate at 20 µg
  Lane 7 : RHM (Rat heart mitochondrial homogenate) at 20 µg
  Lane 8 : HLH (Human liver homogenate) at 20 µg
  Lane 9 : RLH (Rat liver homogenate) at 20 µg
  Lane 10 : MLH (Mouse liver homogenate) at 20 µg
  
  Secondary
  Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 88 kDa
  Observed band size : 88 kDa
  
  
  Exposure time : 1 minute
• 

  Immunocytochemistry/ Immunofluorescence - Anti-CPT1A antibody (ab128568)
  Immunofluorescent staining of CPT1A in H9C2 cells (rat) using ab128568.
  
  Fixation: 4% paraformaldehyde PBS fixed for 20 minutes
  Permebilization: 0.1% Triton X-100 PBS for 30 minutes at room temperature while rocking
  Blocking: 2x Sigma Block 0.1% Triton X-100 H2O for 2 hours at room temperature while rocking
  Primary antibodies: Anti-CPT1A antibody (ab128568) 0.5 ug/mL 1x Sigma Block with 0.1% Triton X-100 incubated overnight at 4 °C.
  Washing: 3x 1% NGS 10 minutes/wash.
  Secondary antibodies: Alexa 488 GAM 1:1000 diluted in 1% NGS with 0.1% Triton X-100 PBS incubated for 2 hours at room temperature while rocking.
  Washing: 3x 1% NGS 10 minutes/wash.
  DAPI: 20 ng/mL in 1% NGS, 0.1% Triton X-100 PBS.
  Washing: 1x 1% NGS 10 minutes/wash.
• 

  Flow Cytometry - Anti-CPT1A antibody [8F6AE9] (ab128568)
  Overlay histogram showing HeLa cells stained with ab128568 (red line) or no primary antibody (black line). The cells were fixed with 4% paraformaldehyde (15 min) and then permeabilized with 0.1% Triton X-100 in PBS, 3% BSA for 10 min. The cells were then incubated in 3% BSA in PBS for 10 minutes to block non-specific protein-protein interactions followed by the antibody (ab128568, 1µg/mL) for 60 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96871) at 1/1000 dilution for 30 min.
• 

  Western blot - Anti-CPT1A antibody [8F6AE9] (ab128568)
  All lanes : Anti-CPT1A antibody [8F6AE9] (ab128568) at 1 µg/ml
  
  Lane 1 : Marker
  Lane 2 : HHH (Human heart homogenate) RIPA Extract Immunoprecipitated with no primary antibody
  Lane 3 : HHH (Human heart homogenate) RIPA Extract Immunoprecipitated with Anti-CPT1A antibody 8F6AE9 (ab128568)
  Lane 4 : HHH (Human heart homogenate) RIPA Extract 20 µg
  
  Secondary
  Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1:10000
  developed using the ECL technique
  
  Performed under non-reducing conditions.
  
  Predicted band size : 88 kDa
  
  
  Exposure time : 1 minute
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [8F6AE9] (ab128568)
  IHC image of CPT1A staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab128568, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
• 

  In-Cell ELISA - Anti-CPT1A antibody [MS#60B 8F6AE9] (ab128568)
  In-Cell ELISA for Anti-CPT1A antibody (ab128568) stained HeLa cells (human)
  
  Seeding: HeLa cells seeded in a 1:2 dilution series starting at 60,000 cells/well across Row A, 30,000 cells/well across Row B, etc. Row H contains no cells.
  Table: Example In-Cell ELISA Average Data from shown plate.
  Fixation: 4% paraformaldehyde PBS fixed for 15 minutes
  Permeabilization: 0.3% Triton X-100 PBS for 30 minutes at room temperature while shaking
  Blocking: 2x Sigma Block 0.3% Triton X-100 H2O for 2 hours at room temperature while shaking
  Primary antibodies: All primaries diluted in 1x Sigma Block with 0.3% Triton X-100 incubated overnight at 4 °C.
  • Columns 1-3: Anti-CPT1A antibody (ab128568) 10 ug/mL
  • Columns 4-6: Anti-CPT1A antibody (ab128568) 1 ug/mL
  • Columns 7-9: Anti-CPT1A antibody (ab128568) 0.1 ug/mL
  • Columns 10-12: No Primary
  Washing: Briefly 4x with 0.3% TWEEN-20 PBS
  Secondary antibodies: Licor GAM 800 1:1000 in 1x Sigma Block 0.3% Triton X-100 H2O incubated for 2 hours at room temperature while shaking.
  Washing: Briefly 5x with 0.3% TWEEN-20 PBS, do not remove last wash.
  Image: Image on Licor with 3.9 focus offset and 7.0 intensity.