Recombinant Anti-CRABP1 antibody [EPR7905] (ab182573)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7905] to CRABP1
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CRABP1 antibody [EPR7905]
See all CRABP1 primary antibodies -
Description
Rabbit monoclonal [EPR7905] to CRABP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Y79, MCF7 and Human skin lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7905 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
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IP |
1/50 - 1/70.
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa). |
IP
1/50 - 1/70. |
Target
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Function
Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1381 Human
- Entrez Gene: 12903 Mouse
- Omim: 180230 Human
- SwissProt: P29762 Human
- SwissProt: P62965 Mouse
- Unigene: 346950 Human
- Unigene: 34797 Mouse
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Alternative names
- Cellular retinoic acid binding protein 1 antibody
- Cellular retinoic acid-binding protein 1 antibody
- Cellular retinoic acid-binding protein I antibody
see all
Images
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Anti-CRABP1 antibody [EPR7905] (ab182573) at 1/1000 dilution + Human skin lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 16 kDa -
Western blot analysis of CRABP1 in immunoprecipitation pellets from Human Y79 lysate. ab182573 used at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
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All lanes : Anti-CRABP1 antibody [EPR7905] (ab182573) at 1/1000 dilution
Lane 1 : Y79 cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 16 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab182573 has not yet been referenced specifically in any publications.