Anti-CRABP2 antibody [10D6] (ab128100)

Overview

• Product nameAnti-CRABP2 antibody [10D6]See all CRABP2 primary antibodies ...
• Description
  Mouse monoclonal [10D6] to CRABP2
• Tested applicationsWB, IHC-P, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length recombinant Human CRABP2 (NP_001869) produced in E.coli.

• Positive controlHuman MCF7 cell lysate and Human ovary tissue. HEK293T cells transfected with pCMV6-ENTRY CRABP2 cDNA.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 50% Glycerol, 1% BSA
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab128100 is purified from mouse ascites fluids by affinity chromatography
• Clonality Monoclonal
• Clone number10D6
• IsotypeIgG1
• Research Areas
  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Retanoic and retanoid

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Retinoic & Retinoid

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Retinoic & Retinoid

Applications

Target

• FunctionTransports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.
• Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
• DomainForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
• Cellular localizationCytoplasm. Nucleus. Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus.

Target information above from: UniProt accession P29373 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1382 Human
  • Omim: 180231 Human
  • SwissProt: P29373 Human
  • Unigene: 405662 Human

• Alternative names
  Cellular retinoic acid binding protein 2 antibodyCellular retinoic acid binding protein II antibodyCellular retinoic acid-binding protein 2 antibody

  Cellular retinoic acid-binding protein II antibodyCRABP II antibodyCRABP-II antibodyCrabp2 antibodyCRABPII antibodyRABP2_HUMAN antibodyRBP6 antibody

  see all

Anti-CRABP2 antibody [10D6] images

• 

  Western blot - Anti-CRABP2 antibody [10D6] (ab128100)
  All lanes : Anti-CRABP2 antibody [10D6] (ab128100) at 1/500 dilution
  
  Lane 1 : Human HepG2 cell lysate
  Lane 2 : Human HeLa cell lysate
  Lane 3 : Mouse SVT2 cell lysate
  Lane 4 : Human A549 cell lysate
  Lane 5 : Monkey COS7 cell lysate
  Lane 6 : Human jurkat cell lysate
  Lane 7 : Canine MDCK cell lysate
  Lane 8 : Rat PC12 cell lysate
  Lane 9 : Human MCF7 cell lysate
  
  Lysates/proteins at 35 µg per lane.
  
  
  Predicted band size : 16 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [10D6] (ab128100)
  ab128100, at 1/150 dilution, staining CRABP2 in paraffin-embedded Adenocarcinoma of Human ovary by Immunohistochemistry.
• 

  Western blot - Anti-CRABP2 antibody [10D6] (ab128100)
  All lanes : Anti-CRABP2 antibody [10D6] (ab128100) at 1/500 dilution
  
  Lane 1 : pCMV6-ENTRY control cDNA lysate
  Lane 2 : pCMV6-ENTRY CRABP2 cDNA lysate
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 16 kDa
  
• 

  Flow Cytometry - Anti-CRABP2 antibody [10D6] (ab128100)
  HEK293T cells transfected with either CRABP2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained with ab128100 at 1/100, and then analyzed by flow cytometry.