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Product name
Anti-CREB antibody - ChIP Grade
See all CREB products (30) ...
Description
Rabbit polyclonal to CREB - ChIP Grade
Specificity
Immunogen has 92% alignment to ATF-1
Tested applications
ChIP, WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide (Human) - non-phosphopeptide derived from human CREB around the phosphorylation site of Serine 133.
Positive control
HeLa cell extract. Human breast carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution. PubMed: 20622856
WB: 1/500 - 1/1000. Predicted molecular weight: 37 kDa.
ELISA
ELISA: 1/20000.ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
Target
Function
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
Involvement in disease
Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.
Post-translational
modifications
Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP16220
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Active transcription factor CREB antibody
- cAMP response element binding protein 1 antibody
- cAMP response element binding protein antibody
see all
Database links
- Entrez Gene: 1385 Human
- Entrez Gene: 12912 Mouse
- Entrez Gene: 81646 Rat
- Omim: 123810 Human
- SwissProt: P16220 Human
- SwissProt: Q01147 Mouse
- SwissProt: P15337 Rat
- Unigene: 516646 Human
see all
Anti-CREB antibody - ChIP Grade images:
Immunohistochemistry (Paraffin-embedded sections) - CREB antibody (ab31387)
Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 ab31387. Left: Untreated sample; Right: Sample treated with synthesized peptide (negative control).
Western blot - CREB antibody (ab31387)
Lane 1 : Anti-CREB antibody - ChIP Grade (ab31387) at 1/500 dilution
Lane 2 : Anti-CREB antibody - ChIP Grade (ab31387) at 1/500 dilution (treated with synthesized peptide )
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract
Predicted band size : 37 kDa
References for Anti-CREB antibody - ChIP Grade (ab31387)
This product has been referenced in:
- Gao J et al. A novel pathway regulates memory and plasticity via SIRT1 and miR-134. Nature 466:1105-9 (2010). WB, ChIP; Mouse.Read more (PubMed: 20622856) »
- Svensson M et al. Heat stabilization of the tissue proteome: a new technology for improved proteomics. J Proteome Res 8:974-81 (2009).Read more (PubMed: 19159280) »
See all 2 publications for this product
Publishing research using ab31387? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"