You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-CREB antibody [E306] (ab32515)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-CREB antibody [E306]
See all CREB products (30) ...

Description

Rabbit monoclonal [E306] to CREB

Specificity

This antibody recognizes CREB, and can also recognize the splice isoform of CREB.

Tested applications

WB, ICC/IF, IHC-P, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Cow

Immunogen

Synthetic peptide (Human), corresponding to residues before DNA binding motif of human CREB.

Positive control

ICC/IF: PC 12 cells. WB: A431 cell lysate. IHC-P: Prostatic carcinoma tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

E306

Isotype

IgG

Research areas

Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper

  • Western blot - CREB antibody [E306] (ab32515)Western blot - CREB antibody [E306] (ab32515) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - CREB antibody [E306] (ab32515)Immunocytochemistry/ Immunofluorescence - CREB antibody [E306] (ab32515) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - CREB antibody [E306] (ab32515)Immunohistochemistry (Paraffin-embedded sections) - CREB antibody [E306] (ab32515) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000. Dete...Read more →

    WB: 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).

  • 1 Image

    ICC/IF

     ICC/IF: 1/250 - 1/500.

    ICC/IF: 1/250 - 1/500.

  • 1 Image

    IHC-P

     IHC-P: 1/250 - 1/500.

    IHC-P: 1/250 - 1/500.

  • 1 Image

    Flow Cyt

     Flow Cyt: 1/40.

    Flow Cyt: 1/40.

  • IP

     IP: 1/80.

    IP: 1/80.

Target

Function

This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.

Involvement in disease

Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.

Sequence similarities

Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.

Post-translational
modifications

Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP16220 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Active transcription factor CREB antibody
  • cAMP response element binding protein 1 antibody
  • cAMP response element binding protein antibody
  • cAMP responsive element binding protein 1 antibody
  • cAMP-responsive element-binding protein 1 antibody
  • CREB 1 antibody
  • CREB antibody
  • CREB-1 antibody
  • CREB1 antibody
  • CREB1_HUMAN antibody
  • Cyclic AMP-responsive element-binding protein 1 antibody
  • MGC9284 antibody
  • OTTHUMP00000163864 antibody
  • OTTHUMP00000163865 antibody
  • OTTHUMP00000206660 antibody
  • OTTHUMP00000206662 antibody
  • OTTHUMP00000206667 antibody
  • Transactivator protein antibody
see all

Database links

see all

Anti-CREB antibody [E306] images:

  Western blot - CREB antibody [E306] (ab32515)

Western blot - CREB antibody [E306] (ab32515)

Anti-CREB antibody [E306] (ab32515) at 1/1000 dilution + A431 cell lysate

Predicted band size : 37 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)

  Immunocytochemistry/ Immunofluorescence - CREB antibody [E306] (ab32515)

Immunocytochemistry/ Immunofluorescence - CREB antibody [E306] (ab32515)

Ab32515, at a 1/500 dilution, staining CREB in PC12 cells by Immunofluorescence.

  Immunohistochemistry (Paraffin-embedded sections) - CREB antibody [E306] (ab32515)

Immunohistochemistry (Paraffin-embedded sections) - CREB antibody [E306] (ab32515)

Ab32515, at a 1/500 dilution, staining CREB in paraffin embedded prostatic carcinoma tissue sections by Immunohistochemistry.

  Flow Cytometry - CREB antibody [E306] (ab32515)

Flow Cytometry - CREB antibody [E306] (ab32515)

Overlay histogram showing HEK293 cells stained with ab32515 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32515, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-CREB antibody [E306] (ab32515)

This product has been referenced in:

  • Polytarchou C  et al. Akt2 regulates all Akt isoforms and promotes resistance to hypoxia through induction of miR-21 upon oxygen deprivation. Cancer Res : (2011).Read more (PubMed: 21555366) »
  • Zeitlin R  et al. Caffeine induces beneficial changes in PKA signaling and JNK and ERK activities in the striatum and cortex of Alzheimer's transgenic mice. Brain Res 1417:127-36 (2011). Mouse.Read more (PubMed: 21907331) »

See all 4 publications for this product

Publishing research using ab32515? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"