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Anti-CREB (phospho S133) antibody [E113] (ab32096)

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Overview

Product name

Anti-CREB (phospho S133) antibody [E113]
See all CREB products (30) ...

Description

Rabbit monoclonal [E113] to CREB (phospho S133)

Specificity

This antibody is specific for CREB phosphorylated on Serine 133.

Tested applications

IHC-P, WB, IHC-P, IP, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Chicken, Cow, Zebrafish

Immunogen

Synthetic phoshopeptide corresponding to residues surrounding Serine 133 of human CREB.

Positive control

WB: A431 cell lysate. IF: A431 cells. IHC-P: Thyroid gland adenocarcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

E113

Isotype

IgG

Research areas

Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper

  • Western blot - CREB (phospho S133) antibody [E113] (ab32096)Western blot - CREB (phospho S133) antibody [E113] (ab32096) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - CREB (phospho S133) antibody [E113] (ab32096)Immunocytochemistry/ Immunofluorescence - CREB (phospho S133) antibody [E113] (ab32096) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CREB (phospho S133) antibody [E113] (ab32096)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CREB (phospho S133) antibody [E113] (ab32096) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/250.
IHC-P: 1/250.
IP: 1/40.
WB: 1/500. Predicted molecular weight: 37 kDa.

Is unsuitable for FACS.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.

Involvement in disease

Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.

Sequence similarities

Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.

Post-translational
modifications

Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP16220 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Active transcription factor CREB antibody
  • cAMP response element binding protein 1 antibody
  • cAMP response element binding protein antibody
  • cAMP responsive element binding protein 1 antibody
  • cAMP-responsive element-binding protein 1 antibody
  • CREB 1 antibody
  • CREB antibody
  • CREB-1 antibody
  • CREB1 antibody
  • CREB1_HUMAN antibody
  • Cyclic AMP-responsive element-binding protein 1 antibody
  • MGC9284 antibody
  • OTTHUMP00000163864 antibody
  • OTTHUMP00000163865 antibody
  • OTTHUMP00000206660 antibody
  • OTTHUMP00000206662 antibody
  • OTTHUMP00000206667 antibody
  • Transactivator protein antibody
see all

Database links

see all

Anti-CREB (phospho S133) antibody [E113] images:

  Western blot - CREB (phospho S133) antibody [E113] (ab32096)

Western blot - CREB (phospho S133) antibody [E113] (ab32096)

All lanes : Anti-CREB (phospho S133) antibody [E113] (ab32096) at 1/500 dilution

Lane 1 : A431 cells untreated.
Lane 2 : A431 cells treated with forskolin.


Predicted band size : 37 kDa
Observed band size : 46 kDa (why is the actual band size different from the predicted?)

  Immunocytochemistry/ Immunofluorescence - CREB (phospho S133) antibody [E113] (ab32096)

Immunocytochemistry/ Immunofluorescence - CREB (phospho S133) antibody [E113] (ab32096)

Ab32096, at a 1/250 dilution, staining CREB in A431 cells by Immunofluorescence.
Image A: Cells are untreated.
Image B: Cells are treated with Phosphatase.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CREB (phospho S133) antibody [E113] (ab32096)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CREB (phospho S133) antibody [E113] (ab32096)

Ab32096, at a 1/250 dilution, staining CREB in paraffin embedded human thyroid gland adenocarcinoma tissue by Immunohistochemistry.
Image A: Cells are untreated.
Image B: Cells are treated with Phosphatase.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CREB (phospho S133) antibody [E113] (ab32096)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CREB (phospho S133) antibody [E113] (ab32096)

ab32096 staining CREB in Rat hippocampus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Sections were formalin-fixed, and subjected to antigen retrieval by autoclave prior to blocking with 8% milk for 30 minutes at 37°C. The primary antibody was diluted 1/100 with DAKO antibody diluent and incubated with the sample for 18 hours at 4°C. An LSAB-labeled Streptavidin-Biotin conjugated Goat polyclonal antibody was used undiluted as the secondary antibody.

This image is courtesy of an Abreivew submitted by Akiko Shingo.

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References for Anti-CREB (phospho S133) antibody [E113] (ab32096)

This product has been referenced in:

  • Shorning BY  et al. Intestinal renin-angiotensin system is stimulated after deletion of Lkb1. Gut : (2011). Mouse.Read more (PubMed: 21813469) »
  • Petrzilka S  et al. Clock Gene Modulation by TNF-{alpha} Depends on Calcium and p38 MAP Kinase Signaling. J Biol Rhythms 24:283-94 (2009).Read more (PubMed: 19625730) »

See all 3 publications for this product

Publishing research using ab32096? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"