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Product name
Anti-CREB (phospho S133) antibody
See all CREB products (30) ...
Description
Rabbit polyclonal to CREB (phospho S133)
Specificity
This antibody detects endogenous levels of CREB only when phosphorylated at Serine 133.
Tested applications
WB, IHC-P, ELISA, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from Human CREB around the phosphorylation site of Serine 133.
Positive control
Hela cells treated with UV.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Predicted molecular weight: 37 kDa.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/10000.ELISA: 1/10000.
IP: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution. PubMed: 19954954
Target
Function
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
Involvement in disease
Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.
Post-translational
modifications
Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP16220
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Active transcription factor CREB antibody
- cAMP response element binding protein 1 antibody
- cAMP response element binding protein antibody
see all
Database links
- Entrez Gene: 1385 Human
- Entrez Gene: 12912 Mouse
- Entrez Gene: 81646 Rat
- Omim: 123810 Human
- SwissProt: P16220 Human
- SwissProt: Q01147 Mouse
- SwissProt: P15337 Rat
- Unigene: 516646 Human
see all
Anti-CREB (phospho S133) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - CREB (phospho S133) antibody (ab30651)
ab30651 staining human CREB in breast carcinoma by Immunohistochemistry, Paraffin embedded tissue.
Left image antibody un-treated.
Right image antibody pre-incubated with synthetic phosphopeptide.
Western blot - CREB (phospho S133) antibody (ab30651)
All lanes : Anti-CREB (phospho S133) antibody (ab30651)
Lane 1 : Hela cells treated with UV. Antibody un-treated.
Lane 2 : Hela cells treated with UV. Antibody pre-incubated with synthetic phosphopeptide.
Predicted band size : 37 kDa
References for Anti-CREB (phospho S133) antibody (ab30651)
This product has been referenced in:
- Brewer GJ et al. Age-related toxicity of amyloid-beta associated with increased pERK and pCREB in primary hippocampal neurons: reversal by blueberry extract. J Nutr Biochem 21:991-8 (2010). ICC/IF; Rat.Read more (PubMed: 19954954) »
- Sadri-Vakili G et al. Cocaine-induced chromatin remodeling increases brain-derived neurotrophic factor transcription in the rat medial prefrontal cortex, which alters the reinforcing efficacy of cocaine. J Neurosci 30:11735-44 (2010). WB; Rat.Read more (PubMed: 20810894) »
See all 4 publications for this product
Publishing research using ab30651? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"