Anti-CREG1 antibody (ab68341)
- Product nameAnti-CREG1 antibodySee all CREG1 primary antibodies ...
- DescriptionMouse monoclonal to CREG1
- Tested applicationsWB, IHC-P, ELISA more details
- Species reactivityReacts with: Human
GST-tagged recombinant fragment, corresponding to amino acids 121-221 of Human CREG1.
- Positive controlHL60 cells; adrenal gland tissue.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2
- Concentration information loading...
- PurityProtein A purified
- Clonality Monoclonal
- Light chain typekappa
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab68341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.|
|IHC-P||IHC-P: Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Pretreatment of sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes is recommended.|
|ELISA||ELISA: Use at an assay dependent dilution.|
- FunctionMay contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R.
- Sequence similaritiesBelongs to the CREG family.
- Cellular localizationSecreted.
- Cellular repressor of E1A stimulated genes 1 antibodyCellular repressor of E1A-stimulated genes 1 antibodyCREG antibody
- CREG1 antibodyCREG1_HUMAN antibodyProtein CREG1 antibody
Anti-CREG1 antibody images
Anti-CREG1 antibody (ab68341) at 5 µg/ml + immunogen at 0.2 µg
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 24 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Anti-CREG1 antibody (ab68341) at 5 µg/ml + HL60 cell lysate at 25 µg
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
ab68341 staining CREG1 on formalin-fixed paraffin-embedded human adrenal gland tissue. Antibody concentration 3 ug/ml. Antigen retrieval in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes was performed.
Detection limit for recombinant GST tagged CREG1 is approximately 0.3ng/ml as a capture antibody.
References for Anti-CREG1 antibody (ab68341)
ab68341 has not yet been referenced specifically in any publications.