Overview

  • Product nameAnti-CREG1 antibody
    See all CREG1 primary antibodies
  • Description
    Mouse monoclonal to CREG1
  • Tested applicationsWB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    GST-tagged recombinant fragment, corresponding to amino acids 121-221 of Human CREG1.

  • Positive control
    • HL60 cells; adrenal gland tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab68341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Pretreatment of sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes is recommended.
ELISA Use at an assay dependent dilution.

Target

  • FunctionMay contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R.
  • Sequence similaritiesBelongs to the CREG family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cellular repressor of E1A stimulated genes 1 antibody
    • Cellular repressor of E1A-stimulated genes 1 antibody
    • CREG antibody
    • CREG1 antibody
    • CREG1_HUMAN antibody
    • Protein CREG1 antibody
    see all

Anti-CREG1 antibody images

  • Anti-CREG1 antibody (ab68341) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 24 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-CREG1 antibody (ab68341) at 5 µg/ml + HL60 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
  • ab68341 staining CREG1 on formalin-fixed paraffin-embedded human adrenal gland tissue. Antibody concentration 3 ug/ml. Antigen retrieval in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes was performed.
  • Detection limit for recombinant GST tagged CREG1 is approximately 0.3ng/ml as a capture antibody.

References for Anti-CREG1 antibody (ab68341)

ab68341 has not yet been referenced specifically in any publications.

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