Overview

  • Product name
  • Description
    Mouse monoclonal to CRLF1
  • Tested applications
    Suitable for: WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: PEKPVNISCW SKNMKDLTCR WTPGAHGETF LHTNYSLKYK LRWYGQDNTC EEYHTVGPHS CHIPKDLALF TPYEIWVEAT NRLGSARSDV LTLDIL, corresponding to amino acids 135-231 of Human CRLF1

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 46 kDa.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • Relevance
    CRLF1 (Cytokine receptor-like factor 1) is a cytokine receptor subunit. It forms a heteromeric complex with cardiotrophin-like cytokine (CLC) and the CRLF1/CLC complex is a ligand for the ciliary neurotrophic factor receptor (CNTFR). Mutations in CRLF1 are responsible for both Crisponi and cold-induced sweating syndromes.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • CISS 1 antibody
    • CISS antibody
    • CISS1 antibody
    • class I cytokine receptor antibody
    • CLF 1 antibody
    • CLF antibody
    • CLF1 antibody
    • CRLF 1 antibody
    • Cytokine like factor 1 antibody
    • Cytokine receptor like factor 1 antibody
    • cytokine type 1 receptor CRLP 1 antibody
    • NR 6 antibody
    • NR6 antibody
    • ZcytoR 5 antibody
    • ZcytoR5 antibody
    see all

Images



  • Predicted band size : 46 kDa
  • Immunoprecipitation of CRLF1 transfected lysate using ab56500. The predicted band size of 46kDa.
  • All lanes : Anti-CRLF1 antibody (ab56500)

    Lane 1 : CRLF1 transfected lysate (293T cell line)
    Lane 2 : Non-transfected lysate


    Predicted band size : 46 kDa

References

This product has been referenced in:
  • Herholz J  et al. Differential secretion of the mutated protein is a major component affecting phenotypic severity in CRLF1-associated disorders. Eur J Hum Genet 19:525-33 (2011). Read more (PubMed: 21326283) »

See 1 Publication for this product

Customer reviews and Q&As

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The clone number is XXXXX.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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