Recombinant Anti-CRMP2 (phospho T514) antibody [EPR7876] (ab129066)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7876] to CRMP2 (phospho T514)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CRMP2 (phospho T514) antibody [EPR7876]
See all CRMP2 primary antibodies -
Description
Rabbit monoclonal [EPR7876] to CRMP2 (phospho T514) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7876 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 62 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 62 kDa). |
Target
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Function
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. -
Post-translational
modifications3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1808 Human
- Entrez Gene: 12934 Mouse
- Entrez Gene: 25416 Rat
- Omim: 602463 Human
- SwissProt: Q16555 Human
- SwissProt: O08553 Mouse
- SwissProt: P47942 Rat
- Unigene: 173381 Human
see all -
Alternative names
- Collapsin response mediator protein 2 antibody
- Collapsin response mediator protein antibody
- Collapsin response mediator protein hCRMP 2 antibody
see all
Images
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All lanes : Anti-CRMP2 (phospho T514) antibody [EPR7876] (ab129066) at 1/1000 dilution
Lane 1 : Human fetal brain lysate, untreated
Lane 2 : Human fetal brain lysate, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 62 kDa
The lower panel was analysed using a monoclonal antibody raised against non-phosphoryated CRMP2.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab129066 has been referenced in 2 publications.
- Tian Y et al. Activation of Transient Receptor Potential Vanilloid 4 Impairs the Dendritic Arborization of Newborn Neurons in the Hippocampal Dentate Gyrus through the AMPK and Akt Signaling Pathways. Front Mol Neurosci 10:190 (2017). WB ; Mouse . PubMed: 28663724
- Zhang H et al. Brain-specific Crmp2 deletion leads to neuronal development deficits and behavioural impairments in mice. Nat Commun 7:N/A (2016). WB ; Mouse . PubMed: 27249678