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Overview

  • Product nameCRMP3 protein (Tagged-His Tag)See all CRMP3 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 258-561 (304aa) of Human CRMP3 with a N terminal His tag; predicted MWt 35 kDa: Uniprot ID = O14531.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:reconstitution with 64 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesKGAADAIAQAKRRGVVVFGEPITASLGTDGSHYWSKNWAK AAAFVTSPPVN PDPTTADHLTCLLSSGDLQVTGSAHCT FTTAQKAVGKDNFALIPEGTNGIE ERMSMVWEKCVASG KMDENEFVAVTSTNAAKIFNFYPRKGRVAVGSDADLV I WNPKATKIISAKTHNLNVEYNIFEGVECRGAPAVVISQGR VALEDGKMFV TPGAGRFVPRKTFPDFVYKRIKARNRLA EIHGVPRGLYDGPVHEVMVPAKP GSGAPARASCPGKIS VPPVRNLHQSGFSLSGSQADDHIARRTAQKIMAP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92000 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Collapsin response mediator protein 3CRMP 3CRMP-3
        CRMP3Dihydropyrimidinase like 4Dihydropyrimidinase related protein 4Dihydropyrimidinase-related protein 4DPYL4_HUMANDPYSL4DRP 4DRP-4DRP4ULIP 4ULIP-4ULIP4UNC33 like phosphoprotein 4UNC33-like phosphoprotein 4
      see all
  • FunctionNecessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
  • Sequence similaritiesBelongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession O14531 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    CRMP3 protein (Tagged-His Tag) images

    • SDS-PAGE - CRMP3 protein (Tagged-His Tag) (ab92000)
      SDS-PAGE - CRMP3 protein (Tagged-His Tag) (ab92000)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for CRMP3 protein (Tagged-His Tag) (ab92000)

    ab92000 has not yet been referenced specifically in any publications.

    Publishing research using ab92000 ? Please let us know so that we can cite the reference in this datasheet

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