|
|
|
|
Western blot - CRMP5 antibody (ab79008)
(enlarge)
|
|
|
|
|
Product Name
|
CRMP5 antibody
|
| |
See all CRMP5 antibodies (5)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to CRMP5
|
|
Immunogen
|
Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human CRMP5.
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Ms, Rat
(Due to sequence homology)
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
This antibody gave a positive signal in human brain and human spinal cord tissue lysates and in the following whole cell lysates: SHSY-5Y; SK N BE; SHSY-5Y.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
|
|
Relevance
|
The collapsin response mediator protein (CRMP) family of proteins (also referred to as ULIP6) are a group of neurologic proteins that have been found to play key roles in growth cone guidance during neural development. CRMP5 has relatively low sequence homology with the other four members of the CRMP family. CRMP5 is expressed in the developing nervous system in an expression pattern that resembles CRMP2 and has a peak expression during the first postnatal week. Elevated levels of autoantibody to CRMP5 have been shown to be linked paraneoplastic autoimmune optic neuritis and other paraneoplastic neoplasms.
Auto-immunity against CRMP5 expression (predominantly the N-terminal epitopes) is also being investigated as an oncological marker in the respiratory system and in the thymus.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CRMP5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for CRMP5 antibody images, references, products related to ab79008 and other tools.
|
| |
CRMP5 antibody images:
|
 Western blot - CRMP5 antibody (ab79008)
All lanes : CRMP5 antibody (ab79008) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)
Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 4 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 61 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa,55 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
CRMP5 contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted.
|
| |
| |
|
Search PubMed (MEDLINE) for references to CRMP5
|
| |
CRMP5 antibody - more information
|
Customer reviews (feedback) regarding CRMP5 antibody |
Customer FAQs regarding CRMP5 antibody |
Protocols for CRMP5 antibody |
Price and availability of products related to CRMP5 antibody |
| |
CRMP5 antibody - related products:
|
|
|
| |
CRMP5 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about CRMP5 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
