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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Overview
Product name
Anti-CRMP5 antibody
See all CRMP5 products (5) ...
Description
Rabbit polyclonal to CRMP5
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human CRMP5.
(Peptide available as ab89373.)
Positive control
This antibody gave a positive signal in human brain and human spinal cord tissue lysates and in the following whole cell lysates: SHSY-5Y; SK N BE; SHSY-5Y.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).
ICC/IF: Use a concentration of 5 µg/ml
Target
Relevance
The collapsin response mediator protein (CRMP) family of proteins (also referred to as ULIP6) are a group of neurologic proteins that have been found to play key roles in growth cone guidance during neural development. CRMP5 has relatively low sequence homology with the other four members of the CRMP family. CRMP5 is expressed in the developing nervous system in an expression pattern that resembles CRMP2 and has a peak expression during the first postnatal week. Elevated levels of autoantibody to CRMP5 have been shown to be linked paraneoplastic autoimmune optic neuritis and other paraneoplastic neoplasms. Auto-immunity against CRMP5 expression (predominantly the N-terminal epitopes) is also being investigated as an oncological marker in the respiratory system and in the thymus.
Cellular localization
Cytoplasmic
Alternative names
- Collapsin response mediator protein 5 antibody
- CRAM antibody
- CRMP 3 associated molecule antibody
see all
Database links
- Entrez Gene: 56896 Human
- Entrez Gene: 65254 Mouse
- Entrez Gene: 65208 Rat
- Omim: 608383 Human
- SwissProt: Q9BPU6 Human
- SwissProt: Q9EQF6 Mouse
- SwissProt: Q9JHU0 Rat
- Unigene: 299315 Human
see all
Anti-CRMP5 antibody images:
Western blot - CRMP5 antibody (ab79008)
All lanes : Anti-CRMP5 antibody (ab79008) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)
Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 4 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 61 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa,55 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
CRMP5 contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted.
Immunocytochemistry/ Immunofluorescence - CRMP5 antibody (ab79008)
ICC/IF image of ab79008 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79008, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) SHSY5Y cells at 5µg/ml.
References for Anti-CRMP5 antibody (ab79008)
ab79008 has not yet been referenced specifically in any publications.
Publishing research using ab79008? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"